Monoclonal Anti-Cytokeratin Peptide 18 antibody produced in mouse
SIGMA/C1399 - clone KS-B17.2, ascites fluid
Synonym: Anti-CK18
MDL Number: MFCD00162250
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human prostate tissue sections were stained with 1:200 Monoclonal Anti-Cytokeratin Peptide 18 antibody produced in Mouse, Clone: KS-B17.2 (Cat. No. C1399) followed by 1:40 Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat (Cat. No. F5262).
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | KS-B17.2, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect immunofluorescence: 1:100 using formalin-fixed, paraffin-embedded sections of human tissue | |
| microarray: suitable | |
| UniProt accession no. | P05783  |
| Application: | Monoclonal Anti-Cytokeratin Peptide 18 antibody produced in mouse has been used in: • immunofluorescence • immunoperoxidase staining • immunocytochemistry • microarray |
| Biochem/physiol Actions: | Cytokeratins are proteins of keratin-containing intermediate filaments that provide mechanical support and other additional functions in epithelial cells. It is found in the intracytoplasmic cytoskeleton of epithelial tissue. Epithelial tissue expresses cytokeratin subunits in a specific and stable pattern. Cytokeratins along with vimentin are involved in cell proliferation, migration and differentiation of preodontoblasts and preameloblasts. The intermediate-sized filaments are abundant in human endothelial cells and are mostly of vimentin type. |
| Biochem/physiol Actions: | The antibody reacts with a wide variety of simple epithelia (gastrointestinal, respiratory, urinary, liver, and glandular) but does not react with most stratified squamous epithelium (esophagus, epidermis) or non-epithelial cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal anti-Cytokeratin Peptide 18 (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. |
| Immunogen: | keratin from the bovine mammary gland epithelial cell line BMGE. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |