Cholesterol Oxidase microbial
SIGMA/C1235 - recombinant, lyophilized powder, ≥10 units/mg protein
Synonym: Cholesterol: oxygen oxidoreductase
CAS Number: 9028-76-6
EC Number: 232-842-1
MDL Number: MFCD00130783
Product Type: Chemical
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| form | lyophilized powder |
| mol wt | 55 kDa |
| Quality Level | 200  |
| solubility | 50 mM potassium phosphate buffer, pH 7.0: soluble |
| specific activity | ≥10 units/mg protein |
| storage temp. | −20°C |
| Application: | Cholesterol oxidase is used to determine serum cholesterol. The enzyme also finds application in the microanalysis of steroids in food samples and in distinguishing 3-ketosteroids from 3b-hydroxysteroids. Transgenic plants expressing cholesterol oxidase are being investigated in the fight against the cotton boll weevil. CHOD has also been used as a molecular probe to elucidate cellular membrane structures. |
| Biochem/physiol Actions: | Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This bifunctional enzyme oxidizes cholesterol to cholest-5-en-3-one in an FAD-requiring step. This is subsequently isomerized to cholest-4-en-3-one with the release of H2O2. Optimum pH of the enzyme is 7.0. Hg2+, Ag+, ionic detergents inhibit the enzyme activity. |
| Other Notes: | One unit will convert 1.0 μmol of cholesterol to 4-cholesten-3-one per minute at 37 °C and pH 7.0 in a peroxidase linked system. |
| Packaging: | 100 units in glass bottle |
| Preparation Note: | Dissolves in cold 50 mM potassium phosphate buffer, pH 7.0. Solution is to be prepared just before use. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥10 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.1.3.6 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |