Anti-Cy3/Cy5 antibody, Mouse monoclonal
SIGMA/C0992 - clone CY-96, purified from hybridoma cell culture
Synonym: Monoclonal Anti-Cy3/Cy5; Cy3 Antibody; Cy3 Antibody - Anti-Cy3/Cy5 antibody, Mouse monoclonal
Product Type: Chemical
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| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | CY-96, monoclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: suitable |
| dot blot: 1-2 μg/mL using cell protein extracts labeded with Cy3 or Cy5 | |
| immunocytochemistry: suitable | |
| immunoprecipitation (IP): suitable | |
| microarray: suitable |
| Application: | Anti-Cy3/Cy5 antibody, Mouse monoclonal has been used in: • immunofluorescence • western blot • dot blot • enzyme linked immunosorbent assay (ELISA) • immunoprecipitation • immunocytochemistry • protein microarrays • In in situ hybridization |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunofluorescence (1 paper) |
| Biochem/physiol Actions: | Cy 3 and Cy5 are the most popular cyanine dyes, used combined for two color detection. Cy3 dyes are fluorescent orange while Cy5 is fluorescent in the red region. Cyanine belonging to polymethine group. CyDyes are a family of fluorophores that can be used for labeling proteins, peptides, DNA, RNA, and other biomolecules. These dyes are small, pH insensitive, soluble in aqueous solution and are tolerant to DMSO. They are more photostable than fluorescein, have high molar extinction coefficients and favorable quantum yields. Mainly Cy3 and Cy5 are used in many different biological assays such as DNA microarrays, protein microarrays, two-dimensional protein analysis (2D gels), fluorescence resonance energy transfer (FRET), and immunocytochemistry. |
| Biochem/physiol Actions: | The antibody recognizes Cy3 and Cy5 conjugated to proteins. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Cy3/Cy5 antibody, Mouse monoclonal, (mouse IgG2a isotype) is derived from the hybridoma CY-96 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from Balb/c mice immunized with a mixture of proteins labeled with Cy3 or Cy5. |
| Immunogen: | mixture of proteins labeled with Cy3/Cy5. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |