Clostripain from Clostridium histolyticum
SIGMA/C0888 - ≥20 units/mg solid
Synonym: Clostridiopeptidase B; Proteinase from Clostridium histolyticum
CAS Number: 9028-00-6
EC Number: 232-822-2
MDL Number: MFCD00130814
Product Type: Chemical
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| form | lyophilized powder |
| impurities | salt, essentially free |
| mol wt | 15.4 kDa |
| 41.7 kDa | |
| Quality Level | 100  |
| specific activity | ≥20 units/mg solid |
| storage temp. | 2-8°C |
| Analysis Note: | Purified, essentially salt-free |
| Analysis Note: | The enzyme must be activated for 2-3 hours before use by dissolving in 2.5 mM dithiothreitol containing 1.0 mM calcium acetate. |
| Application: | Clostripain from Clostridium histolyticum has been used as a proteolytic enzyme in perfusate to detect its effect on tube hematocrit. It has also been used in limited proteolysis of DNA polymerase (gp43) of phage T4 (RB69 gp43). |
| Biochem/physiol Actions: | Clostripain from Clostridium histolyticum is composed of two polypeptide chains, with molecular masses of 41.7 kDa and 15.4 kDa. Clostripain has a highly restricted substrate specificity for Arg-Xaa peptide bonds. Therefore, clostripain has been explored as a potential enzyme for protein sequencing purposes. It has also been studied as a catalyst for condensation of pharmaceutically important peptides containing Arg-Pro bonds. |
| Other Notes: | One unit will hydrolyze 1.0 μmole of BAEE per min at pH 7.6 at 25 °C in the presence of 2.5 mM DTT. |
| Packaging: | Bottomless glass bottle. Contents are inside inserted fused cone. |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H334 - H335 |
| Precautionary statements | P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥20 units/mg solid |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.4.22.8 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |