Anti-Cullin 3 antibody produced in rabbit
SIGMA/C0871 - ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-CUL3
MDL Number: MFCD03454920
Product Type: Chemical
Immunofluorescence 3T3 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.1% Triton X-100. Fixed cells were stained with 10 μg/mL Rabbit Anti-Cullin 3 (Cat. No. C0871). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.
Immunoprecipitation Rabbit Anti-Cullin 3 (Cat. No. C0871) was used to immunoprecipitate Cullin 3 from rat brain whole extract. Detection antibody: Rabbit Anti-Cullin 3 (C-terminal) antibody (Cat. No. C9745). Lanes 1. 5 μg antibody 2. Negative control: 5 μg non-relevant antibody 3. Negative control: without precipitating antibody
Immunoblotting Whole extract of HEK-293T cells overexpressing human Cullin 3 was separated on SDS-PAGE and probed with Rabbit Anti-Cullin 3 (Cat. No. C0871). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. 2 μg/mL antibody 3. 2 μg/mL antibody + Cullin 3 immunizing peptide (human, 194-211)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~80 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 5-10 μg using lysate of rat brain |
| indirect immunofluorescence: 5-10 μg/mL using mouse 3T3 cells | |
| western blot: 1-2 μg/mL using whole extract of HEK-293T cells expressing human Cullin 3 | |
| UniProt accession no. | Q13618  |
| Application: | Anti-Cullin 3 antibody produced in rabbit has been used in • western blotting • immunoprecipitation • immunofluorescence |
| Biochem/physiol Actions: | Cullin 3 (Cul3) acts as a key mediator of post-translational protein regulation. It functions as a scaffold protein that assemble multi-subunit ubiquitin ligase complexes which are responsible for the specific recognition and targeting of substrates for ubiquitin-dependent degradation by the 26S proteasome. Cul3 is required for degradation of the microtubule-severing protein MEI-1/katanin during transitioning from meiosis-to-mitosis phase. It forms a catalytically inactive BTB-Cul3-Rbx1 (BCR) ubiquitin ligase and becomes functional upon covalent attachment of the ubiquitin homologue neural-precursor-cell-exp |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Cullin 3 is a member of the cullin family of E3 ubiquitin protein ligases. Cullin 3 forms the BTB-Cul3-Rbx1 (BCR) ubiquitin ligase complex that contains a RING-box protein, Rbx1, and a BTB containing protein. Rbx1 binds to the C-terminus of cullin 3 through its RING-type zinc finger domain to recruit a ubiquitin-conjugating enzyme. The BTB containing protein binds cullin 3 at its N-terminus and functions as a cullin specific adaptor protein. |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |