Cyclooxygenase 2 human
SIGMA/C0858 - ≥70% (SDS-PAGE), recombinant, expressed in insect cells, aqueous solution, ≥8000 units/mg protein
Synonym: COX-2; Prostaglandin H synthase 2
MDL Number: MFCD00071378
Product Type: Chemical
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| assay | ≥70% (SDS-PAGE) |
| form | aqueous solution |
| mol wt | dimer subunit mol wt 70-74 kDa |
| Quality Level | 200  |
| recombinant | expressed in insect cells |
| expressed in insect cells Active recombinant N-terminal His-tagged COX-2 N580A mutant purified from insect cells. | |
| shipped in | dry ice |
| specific activity | ≥8000 units/mg protein |
| storage temp. | −70°C |
| UniProt accession no. | P35354  |
| Biochem/physiol Actions: | COX-2 catalyzes the conversion of arachidonic acid to prostaglandin H2 (the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins). It is the inducible form of cyclooxygenase, induced by phorbol esters, lipopolysaccharides, and cytokines. COX-2 is upregulated in the neovasculature of malignant tumors. Inhibition of COX-2 with nonsteroidal anti-inflammatory drugs (NSAIDs) is associated with decreased tumor growth. |
| General description: | KM for arachidonate = 6.5 μM. Exhibits activity and sensitivity to NSAIDs similar to the non-tagged enzyme. |
| Legal Information: | TWEEN is a registered trademark of Croda International PLC |
| Other Notes: | One unit will consume 1.0 nanomole of oxygen per minute at pH 8 at 37 °C. |
| Other Notes: | Tagged with a six-residue histidine sequence near the N-terminus. |
| Physical form: | Solution in 80 mM Tris, pH 8.0, with 0.3 mM DDC, 0.01% TWEEN® 20 and 10% glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: ≥8000 units/mg protein |
| Storage Temp. | −70°C |
| UNSPSC | 12352204 |