Chitosanase from Streptomyces sp.
SIGMA/C0794 - buffered aqueous glycerol solution, ≥15 units/mg protein (E1%)
Synonym: Chitosan N-
CAS Number: 51570-20-8
MDL Number: MFCD00211797
Product Type: Chemical
Chitosanase specificity: Chitosan is a polymer of β-(1-4)-N--acetyl-D-glucosamine units.
| concentration | ≥0.5 mg/mL |
| form | buffered aqueous glycerol solution |
| Quality Level | 200  |
| solubility | 0.1 M acetate buffer, pH 5.5: soluble |
| specific activity | ≥15 units/mg protein (E1%) |
| storage temp. | −20°C |
| Application: | Chitosanase from Sigma has been used for polarized light-stimulated, enzymatic hydrolysis of chitin and chitosan. |
| Biochem/physiol Actions: | Chitosanase catalyzes the endohydrolysis of β-1,4-linkages between D-glucosamine (GlcN-GlcN) residues in chitosan. The enzyme from Streptomyces has been reported to also hydrolyze the GlcNAc-GlcN linkage in partially acetylated chitosan. |
| Other Notes: | One unit will liberate 1.0 μmole of reducing sugar (measured as |
| Other Notes: | View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enz |
| Physical form: | Solution in 50% glycerol containing 50 mM sodium acetate |
| Preparation Note: | Soluble in 10mL of 0.1 M Acetate buffer at pH 5.5 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥15 units/mg protein (E1%) |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.2.1.132 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |