Crystal Violet
SIGMA/C0775 - certified by the BSC
Synonym: Basic Violet 3; Gentian Violet; Hexamethylpararosaniline chloride; Methyl Violet 10B
CAS Number: 548-62-9
Empirical Formula (Hill Notation): C25H30ClN3
Molecular Weight: 407.98
EC Number: 208-953-6
MDL Number: MFCD00011750
Linear Formula: C25H30N3Cl
Product Type: Chemical
FT-IR Spectra for Basic Violet 3.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C0775-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C0775-25G
| agency | certified by the BSC |
| antibiotic activity spectrum | fungi |
| application(s) | diagnostic assay manufacturing hematology histology |
| color | green to very dark green |
| composition | Dye content, ≥90% |
| density | 1.19 g/cm3 at 20 °C |
| form | powder |
| InChI | 1S/C25H30N3.ClH/c1-26(2)2 |
| InChI key | ZXJXZNDDNMQXFV-UHFFFAOYSA |
| mode of action | cell membrane | interferes |
| enzyme | inhibits | |
| mp | 205 °C (dec.) (lit.) |
| pH | 2.5 — 3.5 (20 °C) |
| Quality Level | 200  |
| SMILES string | [Cl-].CN(C)c1ccc(cc1)C(c |
| solubility | H2O: 1 mg/mL |
| storage temp. | room temp |
| technique(s) | microbe id | staining: suitable |
| Application: | Certified for use with the Gram stain for bacteria, Flemming triple stain with iodine for chromosomes, Lieb′s amyloid stain and Newton′s crystal violet-iodine technique for chromatin and nucleoli. |
| Application: | Crystal violet is used as an active component, primary stain, of Gram stain for differentiation of Gram-negative versus Gram-positive bacteria. It has been used to check cell viability. It has also been used for the staining of cells to study cell migration and invasion. |
| Biochem/physiol Actions: | Crystal violet can be used for DNA visualization in agarose gels. The dye is used only in the presence of high concentrations of DNA. Crystal violet is also used for the staining of bacteria in gram staining technique. It is also used for the staining of plant chromosomes. Crystal Violet also helps in colorimetric measurement of cell viability. |
| Packaging: | 25, 100 g in glass bottle |
| Symbol |     GHS05,GHS07,GHS08,GHS09 |
| Signal word | Danger |
| Hazard statements | H302 - H318 - H351 - H410 |
| Precautionary statements | P202 - P273 - P280 - P301 + P312 - P305 + P351 + P338 - P308 + P313 |
| Hazard Codes | T,N |
| Risk Statements | 45-22-41-50/53 |
| Safety Statements | 53-45-60-61 |
| RIDADR | UN 3077 9 / PGIII |
| WGK Germany | WGK 3 |
| mp | 205 °C (dec.) (lit.) |
| Density | 1.19 g/cm3 at 20 °C |
| Storage Temp. | room temp |
| Colour Index Number | 42555 |
| UNSPSC | 12171500 |