Cyclooxygenase 1 from sheep
SIGMA/C0733 - glycerol solution, ≥1500 units/mg protein
Synonym: COX-1; Constitutive cyclooxygenase; Prostaglandin H synthase 1; Prostaglandin endoperoxide synthase
MDL Number: MFCD02098124
Product Type: Chemical
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| form | glycerol solution |
| mol wt | dimer subunit mol wt 70 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| specific activity | ≥1500 units/mg protein |
| storage temp. | −70°C |
| UniProt accession no. | O78749  |
| Application: | Cyclooxygenase 1 from sheep has been used as positive control protein in western blot analysis of osteoarthritis samples and in prostaglandin synthase activity assay. |
| Biochem/physiol Actions: | COX-1 catalyzes the conversion of arachidonic acid to prostaglandin H2 (the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins). It is involved in the homeostatic role of eicosanoids and constitutively expressed in almost all animal tissues. Has an apparent KM of 8.3 μM for arachidonic acid. |
| General description: | Cyclooxygenase 1 is a 71 kDa membrane bound protein predominantly present in endoplasmic reticulum. It has three domains, the epidermal growth factor (EGF) like domain, enzymatic and membrane binding domain. Cyclooxygenase 1 mediates prostaglandin synthesis and is modulated by anti inflammatory nonsteroidal drugs. |
| Legal Information: | TWEEN is a registered trademark of Croda International PLC |
| Other Notes: | One unit consumes one nanomole of oxygen per minute at 37 °C in 0.1 M Tris-HCl buffer, pH 8, containing 100 μM arachidonate, 5 mM EDTA, 2 mM phenol, and 1 μM hematin. |
| Physical form: | Solution in 80 mM Tris-HCl, pH 8, with 0.1% TWEEN® 20, 300 μM diethyldithiocarbamate and 10% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥1500 units/mg protein |
| Storage Temp. | −70°C |
| Enzyme Commission (EC) Number | 1.14.99.1 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |