Monoclonal Anti-HNK-1/N-CAM (CD57) antibody produced in mouse
SIGMA/C0678 - clone VC1.1, ascites fluid, buffered aqueous solution
Synonym: Monoclonal Anti-HNK-1; Anti-CD57; Anti-N-CAM
MDL Number: MFCD00164634
Product Type: Chemical
Immunohistochemistry
Triple sections from E4 chick embryo: Nervous system (pink) is stained with Monoclonal Anti-HNK-1/N-CAM, clone no. VC1.1 (Cat. No. C0678) and Goat Anti-Mouse IgG-Peroxidase (Cat. No. A3673). Nuclei that incorporated BrdU are stained with Monoclonal Anti-BrdU, clone no. BU-33 (Cat. No. B2531) and Goat Anti-Mouse IgM-Alkaline Phosphatae (Cat. No. A7784). BrdU-negative nuclei were stained with Harris′ hematoxylin (Cat. No. H5522). From Dr. R.S. Goldstein, Dept. of Life Sciences, Bar-Ilan university, Ramat Gan, Israel.
Triple sections from E4 chick embryo: Nervous system (pink) is stained with Monoclonal Anti-HNK-1/N-CAM, clone no. VC1.1 (Cat. No. C0678) and Goat Anti-Mouse IgG-Peroxidase (Cat. No. A3673). Nuclei that incorporated BrdU are stained with Monoclonal Anti-BrdU, clone no. BU-33 (Cat. No. B2531) and Goat Anti-Mouse IgM-Alkaline Phosphatae (Cat. No. A7784). BrdU-negative nuclei were stained with Harris′ hematoxylin (Cat. No. H5522). From Dr. R.S. Goldstein, Dept. of Life Sciences, Bar-Ilan university, Ramat Gan, Israel.
Immunohistochemistry
Cross-section through E3 chick embryo at the level of the wings. The entire nervous system is stained with Monoclonal Anti-HNK-1/N-CAM, clone No. VC1.1, (Cat. No. C0678) using Anti-Mouse IgG (whole molecule)-Peroxidase (Cat. No. A9044) and SIGMA FASTTM DAB tablets as detection system.
From Dr. R.S. Goldstein, Dept. of Life Sciences, Bar-Ilan University, Ramat Gan, Israel.
Cross-section through E3 chick embryo at the level of the wings. The entire nervous system is stained with Monoclonal Anti-HNK-1/N-CAM, clone No. VC1.1, (Cat. No. C0678) using Anti-Mouse IgG (whole molecule)-Peroxidase (Cat. No. A9044) and SIGMA FASTTM DAB tablets as detection system.
From Dr. R.S. Goldstein, Dept. of Life Sciences, Bar-Ilan University, Ramat Gan, Israel.
Western Blotting Whole extract of Rat brain were separated on SDS-PAGE and probed with Monoclonal Anti-HNK-1/N-CAM (CD57) antibody produced in Mouse, Clone: VC1.1 (Cat. No. C0678). The antibody was developed with 1:10,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 1:8,000 antibody 2. 1:4,000 antibody 3. Negative Control
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | VC1.1, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| form | buffered aqueous solution |
| isotype | IgM |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, rat, feline, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1:4,000 using cerebral cortex extract |
| UniProt accession no. | Q9P2W7  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunohistochemistry (1 paper) |
| Application: | Monoclonal Anti-HNK-1/N-CAM (CD57) antibody produced in mouse has been used: • as primary antibody for western blot analysis • immunocytochemistry • immunocytochemical • immunohistological staining |
| Biochem/physiol Actions: | HNK1 (human natural killer-1) is referred as B3GAT1, NK1, CD57, LEU7, NK-1, GLCATP and GLCUATP. It is a member of the glucuronyltransferase gene family. Human natural killer-1 (HNK1) carbohydrate epitope is expressed on various cell adhesion molecules in the nervous system and plays a role in cell-cell and cell-substrate interactions. HNK-1 epitope stabilizes GluR2 on neuronal surface membranes and regulates cell surface stability of GluR2 by modulating the interaction with N-cadherin. It is also plays a role in cell migration and synaptic plasticity. HNK-1 epitope has been associated with the risk of metastasis. It is observed to be highly expressed in melanoma cell lines. |
| Biochem/physiol Actions: | Recognizes the CD57/HNK-1 human myeloid cell associated surface glycoprotein. The epitope recognized is an N-linked carbohydrate which is present in a variety of glycoproteins and in some glycolipids. It is resistant to formalin fixation and paraffin embedding. VC1.1 antibody and the HNK-1 (Leu7) antibody inhibit the binding of each other. The antibody recognizes myelin associated glycoprotein in some species and a high molecular weight chondroitin sulphate proteoglycan. 5th Workshop: code no. NK 67 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | CD57/HNK-1 is expressed on a subpopulation of 15-20% of peripheral blood mononuclear cells, about 60% of NK active cells and on a subset of T cells. |
| General description: | Monoclonal Anti-HNK-1/N-CAM (CD57) (mouse IgM isotype) is derived from the VC1.1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Human natural killer cell -1 (HNK-1), also referred as B3GAT1, NK1, CD57, LEU7, NK-1, GLCATP and GLCUATP, is mapped to human chromosome 11q25. The encoded protein belongs to the glucuronyltransferase gene family. Neural cell-adhesion molecule (N-CAM) is expressed on all neuroepithelial cells from the time of neural induction in the early embryo. It is known that the sulfated carbohydrate HNK-1 epitope is carried by several surface molecules including a proportion of N-CAM isoforms. |
| Immunogen: | A homogenate of cat primary visual cortex (area 17) |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |