Anti-Calsequestrin-1 antibody produced in rabbit
SIGMA/C0618 - ~1.6 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Aspartactin; Anti-CALMITINE; Anti-CASQ1; Anti-
MDL Number: MFCD09265355
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-Calsequestrin-1 antibody produced in Rabbit (Cat. No. C0618) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing Calsequestrin-1 (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 1:3,000.
Immunoblotting Tissue extracts and whole cell lysates were separated on SDS-PAGE and blotted with Anti-Calsequestrin-1 (Cat. No. C0618). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and chemiluminescent substrate. Antibody dilution 1:3,000 Lanes 1: rat skeletal muscle (S1 fraction) 2: HEK-293T cells transfected with human calsequestrin-1 3: HEK-293T cells transfected with human calsequestrin-2 4: untransfected HEK-293T cells 5: rat skeletal muscle (S1 fraction) + Inhibition with calsequestrin-1 immunizing peptide (mouse 66-84) (10 μg/mL)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.6 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~60 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | canine, mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 0.5-1 μg/mL using extracts of rat skeletal muscle S1 fraction |
| UniProt accession no. | P31415  |
| Application: | Anti-Calsequestrin-1 antibody produced in rabbit has been used in immunofluorescence. |
| Application: | Anti-Calsequestrin-1 antibody produced in rabbit is suitable for western blotting at a concentration of 0.5-1μg/mL using extracts of rat skeletal muscle S1 fraction. |
| Biochem/physiol Actions: | Calsequestrin-1 is a putative auto-antigen associated with eye muscle inflammation in Graves′ disease. Calsequestrin-2 (55 kDa) is found in the SR′s terminal cisternae luminal space of cardiac, slow skeletal muscle cells and may be in platelets. The major Ca2+ binding protein in cardiac and skeletal muscle is a high-capacity, low-affinity Ca2+ binding glycoprotein, which functions as an internal Ca2+ store in the lumen of the SR. It helps in controlling the smooth-muscle contraction. Calsequestrin is expected to modulate ryanodine receptor (RyR) signaling and Ca2+ release with the help of triadin and junction proteins.† |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Calsequestrin (CS also known as CSQ), the major Ca2+ binding protein in cardiac and skeletal muscle, is a high-capacity, low-affinity Ca2+ binding glycoprotein, which function as an internal Ca2+ store in the lumen of the SR.1-3 In mammals, two forms of the protein exist, calsequestrin-1 (CASQ-1) and calsequestrin-2 (CASQ-2), which encode the fast-twitch skeletal muscle and cardiac calsequestrin, respectively. Calsequestrin-1 is found in the SRs terminal cisternae luminal space of fast skeletal muscle cells. Calsequestrin-1 is a putative autoantigen associated with eye muscle inflammation in Graves′ disease. |
| General description: | Calsequestrin is expected to modulate ryanodine receptor (RyR) signaling and Ca2+ release with the help of triadin and junction proteins. |
| Immunogen: | synthetic peptide corresponding to amino acids 66-84 located near the N-terminus of mouse calsequestrin-1, conjugated to KLH. This sequence is identical in human, rat, and dog calsequestrin-1 and highly conserved in bovine (single amino acid substitution). This sequence is not found in calsequestrin-2. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |