Chloroform:Isoamyl alcohol 24:1
SIGMA/C0549 - suitable for nucleic acid purification
Synonym: chloroform-isoamyl alcohol
Product Type: Chemical
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| application(s) | agriculture |
| contains | 0.6-1.0% ethanol as stabilizer |
| form | liquid |
| Quality Level | 200  |
| suitability | suitable for nucleic acid purification |
| Application: | Chloroform:Isoamyl alcohol 24:1 has been used: • to extract bacterial genomic DNA, DNA from Daphnia spp, and salmon sperms. • for a single DNA precipitation step for high-quality DNA extraction. • for protein extraction and RNAse treatment. • in phenolic oxidation to separate phenolics from the aqueous portion. • for RNA isolation from brown adipose tissue (BAT), inguinal white adipose tissue (iWAT), and epididymal adipose tissue (eWAT). • in modified cetyltrimethyl ammonium bromide (CTAB) method to extract total RNA from Impatiens balsamina. |
| General description: | Chloroform:Isoamyl Alcohol 24:1 is an organic reagent used for DNA extraction and precipitation. |
| Symbol |    GHS05,GHS06,GHS08 |
| Signal word | Danger |
| Hazard statements | H302 - H315 - H318 - H331 - H336 - H351 - H361d - H372 |
| Precautionary statements | P280 - P301 + P312 - P302 + P352 - P304 + P340 + P311 - P305 + P351 + P338 - P308 + P313 |
| Hazard Codes | Xn |
| Risk Statements | 63-20/22-36/38-40-48/20/22 |
| Safety Statements | 26-36/37 |
| RIDADR | UN 1888 6.1 / PGIII |
| WGK Germany | WGK 3 |
| Flash Point(F) | 185.0 °F |
| Flash Point(C) | 85 °C |
| UNSPSC | 12190000 |