Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane
SIGMA/C0543 - Type IV (Miller), lyophilized powder, BioReagent, suitable for cell culture
Synonym: Type IV collagen; EHS-collagen
CAS Number: 9007-34-5
EC Number: 232-697-4
MDL Number: MFCD00130825
Product Type: Chemical
Neurite outgrowth of PC12 rat neuronal cells after 2-day growth on ECM Gel Matrix.
Bioassay EC50 is defined as the effective concentration of Collagen that elicits a 50% attachment factor in cell based bioassay.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: C0543-1VL
| biological source | mouse (Engelbreth-Holm-Swarm murine sarcoma basement membrane) |
| form | lyophilized powder |
| packaging | glass bottle of 1 vial |
| product line | BioReagent |
| Quality Level | 200  |
| shipped in | dry ice |
| solubility | 0.25% acetic acid: 0.5-2.0 mg/mL (Keep at 2-8 °C for several hours, occasionally swirling.) |
| storage temp. | −20°C |
| surface coverage | 6‑10 μg/cm2 |
| technique(s) | cell culture | mammalian: suitable |
| type | Type IV (Miller) |
| UniProt accession no. | B1AVK5  |
| P02463 |
|
| P08122 |
|
| Q63ZW6 |
|
| Q9QZS0 |
| Application: | Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane has been used: • in adhesion assays. • in hydrogel fabrication. • to coat cell culture plates for cell differentiation. • to treat round plastic coverslips for scanning electron microscopy (SEM) analysis in an interaction study between A. culbertsoni trophozoites and Schwann cells. |
| Application: | Recommended for use as a cell culture substratum at 6-10 μg/cm2. |
| Biochem/physiol Actions: | Collagen isolated from Engelbreth-Holm-Swarm murine sarcoma basement membrane helps in the development of 3D nodules. It is also used in 2D coatings. |
| General description: | Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types. |
| General description: | Collagen is classified into several structurally and genetically distinct types. Type IV collagen is a predominant structural protein of the basement membrane. It is notably characteristic of the Engelbreth-Holm-Swarm (EHS) mouse sarcoma. It is commercially available as matrigel and cultrex. Matrigel is made of type 4 collagen, laminin, heparan sulfate proteoglycan and entactin obtained from engelbreth-holm-swarm murine sarcoma. The target cells for attachment include: epithelial cells, endothelial cells, muscle cells, and nerve cells. |
| Other Notes: | Native collagen |
| Other Notes: | We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma-type designations with recognized collagen classification types. |
| Packaging: | 1 vial in glass bottle |
| Preparation Note: | 0.75 mg/vial |
| Preparation Note: | Chloroform treated. Pepsin is not used in the preparation. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |