Anti-Calcium Sensing Receptor antibody, Mouse monoclonal
SIGMA/C0493 - clone HL1499 (6D4-3F4-6C4), purified from hybridoma cell culture
Synonym: Anti-CAR; Anti-EIG8; Anti-FHH; Anti-FIH; Anti-GPRC2A; Anti-HHC; Anti-HHC1; Anti-HYPOC1; Anti-NSHPT; Anti-PCAR1; Anti-hCasR
MDL Number: MFCD02262635
Product Type: Chemical
Immunoblotting Rat kidney extract was separated on SDS-PAGE and probed with Monoclonal PA to Calcium Sensing Receptor (MO) CL:HL1499 (6D4-3F4-6C4) (Cat. No. C0493). The antibody was developed with HRP APA Mouse Fab ads/HIgG (gt) conjugate (Cat. No. A9917) and a ECL substrate. Antibody concentration 3 μg/mL
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | HL1499 (6D4-3F4-6C4), monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rabbit, rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| indirect ELISA: suitable | |
| western blot: 3-6 μg/mL using total extract from rat kidney | |
| UniProt accession no. | P41180  |
| Application: | Monoclonal anti-calcium sensing receptor antibody produced in mouse has been used in western blotting and immunohistochemistry. |
| Application: | Monoclonal Anti-Calcium Sensing Receptor antibody produced in mouse is suitable for the following applications: • Indirect ELISA • Immunoblotting • Immunocytochemistry • Immunohistochemistry (formalin-fixed, paraffin-embedded sections) • Western blotting at a concentration of 3-6μg/mL using total extract from rat kidney |
| Biochem/physiol Actions: | Calcium sensing receptor (CASR) regulates cell activity according to the extracellular calcium concentration. It also plays a major role in controlling parathyroid hormone (PTH) secretion. Thus, this protein can be considered as a potent target for therapeutic agents designed to modify parathyroid gland function. CASR has a role in key signal transduction pathways in several tissues such as parathyroid gland, kidney, gastrointestinal tract, placenta, pancreas and brain. Mutation in the gene is associated with autosomal dominant hypocalcemic, hypercalciuria and Bartter syndrome. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The calcium sensing receptor (CASR) gene with 11 exons is mapped to human chromosome 3q13.33-q21.1. CASR is a member of the C family of G protein–coupled receptors and is mainly localized in the parathyroid gland and the thick ascending limb of the Henle loop. CASR is a 1078 amino acid protein. |
| Immunogen: | peptide corresponding to amino-acids 15-29 at the extracellular N-terminus of calcium sensing receptor. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |