BES

SIGMA/B9879 - ≥99.0% (titration)

Synonym: 2-(bis(2-hydroxyethyl)amino)ethane sulfonic acid; n,n-bis[2-hydroxyethyl]-2-aminoethanesulfonic acid; N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid; N,N-Bis(2-hydroxyethyl)taurine

CAS Number: 10191-18-1
Empirical Formula (Hill Notation): C₆H₁₅NO₅S
Molecular Weight: 213.25
EC Number: 233-465-5
MDL Number: MFCD00007533
Linear Formula: C₆H₁₅NO₅S
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-B9879-25G	25 g	$83.10 1/EA	Add To Favorites
45-B9879-100G	100 g	$167.00 1/EA	Add To Favorites
45-B9879-250G	250 g	$366.00 1/EA	Add To Favorites
45-B9879-1KG	1 kg	$1060.00 1/EA	Add To Favorites

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Properties
Descriptions
Safety Info.
Basic Data

application(s)	clinical research diagnostic assay manufacturing general analytical life science and biopharma
assay	≥99.0% (titration)
color	white
density	1.30 g/cm³ at 20—25 °C
	1.35 g/cm³ at 20—25 °C
form	crystalline
InChI	1S/C6H15NO5S/c8-4-1-7(2-5-9)3-6-13(10,11)12/h8-9H,1-6H2,(H,10,11,12)
InChI key	AJTVSSFTXWNIRG-UHFFFAOYSA-N
pH	2.5-5.0 (25 °C, 213.3 g/L)
pKa (25 °C)	7.1
Quality Level	400
SMILES string	OCCN(CCO)CCS(O)(=O)=O
solubility	water: 0.5 g/mL, clear, colorless
storage condition	dry at room temperature
storage temp.	room temp
useful pH range	6.4-7.8

Application:	BES may be employed as binding buffer in modified Eagle′s medium during the binding assay of human melanoma cells. It may be used as biobuffer to investigate the aqueous medium self-assembly of heterometallic CuII/Li 3D coordination polymers.
Application:	Further, BES has found application in transfection studies, both in the preparation of reagents for transfection and in signaling assays related to Neural Melanocortin Receptors.
Features and Benefits:	• Suitable for Cell Culture media and as a Buffering solution in Biochemical and Molecular Biology Research • Effective Buffering from pH 2.5-5.0 (25 °C, 213.3 g/L) with a pKa of 7.1 (25 °C) • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
General description:	N,N-Bis(2-hydroxyethyl)-2<WBR>-aminoethanesulfonic acid, known as BES, is a zwitterionic buffer and a valuable secondary standard in biochemical research. BES, a sulfonic acid-containing cross-linking agent, induces cross-linking between sulfonated polyimide chains in sulfonated polyimide membranes. The useful pH range for BES buffer is 6.4 to 7.8. It is one of the "Good" buffers developed in the 1960s to provide buffering capacity in the pH range of 6.15 to 8.35, making it widely applicable in biochemical studies. BES buffer is suitable for use in systems where the formation of a calcium phosphate-DNA complex is desirable (Absorbance: < 0.05 at 260 nm, 100 mM). Further, BES buffer can be employed in the study of molecular biology, biological buffers, molecular biology reagents, and zwitterionic compounds.
Other Notes:	For additional information on our range of Biochemicals , please complete this form .
Packaging:	1 kg in poly bottle
Packaging:	25, 100, 250 g in poly bottle

Hazard Codes	Xi
Risk Statements	36/37/38
Safety Statements	26
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥99.0% (titration)
Density	1.30 g/cm³ at 20—25 °C; 1.35 g/cm³ at 20—25 °C
Storage Temp.	room temp
UNSPSC	12161700

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