1-Bromo-3-chloropropane
SIGMA/B9673 - for isolation of RNA
Synonym: 1-
CAS Number: 109-70-6
Empirical Formula (Hill Notation): C3H6BrCl
Molecular Weight: 157.44
EC Number: 203-697-1
MDL Number: MFCD00000998
Linear Formula: Cl(CH2)3Br
Product Type: Chemical
| assay | ≥98.5% (GC) |
| bp | 144-145 °C (lit.) |
| density | 1.592 g/mL at 25 °C (lit.) |
| form | liquid |
| grade | Molecular Biology |
| InChI | 1S/C3H6BrCl/c4-2-1-3-5/h1 |
| InChI key | MFESCIUQSIBMSM-UHFFFAOYSA |
| Quality Level | 200  |
| refractive index | n |
| SMILES string | ClCCCBr |
| storage temp. | room temp |
| Application: | 1-Bromo-3-chloropropane has been used in molecular biology applications for the isolation of high quality RNA from: • feline teeth • mice brain and liver tissues • human ectocervical epithelial cells • placental samples • human mesenchymal stromal cells (hMSCs) |
| Features and Benefits: | • less toxic alternative to chloroform • compatible with TRI reagent for isolation of RNA with decreased DNA contamination |
| General description: | BCP can be used in place of chloroform and is less toxic. The use of BCP in the single-step TRI reagent method decreases the possibility of contaminating RNA with DNA. It does not adversely affect quality or quantity of the isolated RNA. 1-Bromo-3-chloropropane is a phase separation reagent used in protocols for isolation of nucleic acids. |
| Other Notes: | BCP can be used in place of chloroform, and is less toxic. The use of BCP in the single-step TRI Reagent method decreases the possibility of contaminating RNA with DNA. It does not adversely affect quality or quantity of the isolated RNA. |
| Packaging: | 200 mL in glass bottle |
| Symbol |   GHS06,GHS08 |
| Signal word | Danger |
| Hazard statements | H302 - H331 - H335 - H341 - H350 - H360FD - H412 |
| Precautionary statements | P201 - P273 - P301 + P312 + P330 - P304 + P340 + P311 - P308 + P313 |
| Hazard Codes | Xn |
| Risk Statements | 10-20/22 |
| Safety Statements | 16 |
| RIDADR | UN 2688 6.1 / PGIII |
| WGK Germany | WGK 2 |
| Flash Point(F) | > 284.0 °F |
| Flash Point(C) | > 140 °C |
| Purity | ≥98.5% (GC) |
| bp | 144-145 °C (lit.) |
| Density | 1.592 g/mL at 25 °C (lit.) |
| Refractive Index | n |
| Storage Temp. | room temp |
| UNSPSC | 41105515 |