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Aniline Blue solution

SIGMA/B8563 - 2.5% in 2% acetic acid

Synonym: Acid blue 93; Cl 42780

CAS Number: 28631-66-5
Empirical Formula (Hill Notation): C32H25N3O9S3Na2
Molecular Weight: 737.73
MDL Number: MFCD00036130
Linear Formula: C32H25N3O9S3Na2
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-B8563-250ML 250 mL
 $71.20
1/EA		 Add To Favorites
45-B8563-500ML 500 mL
 $130.00
1/EA		 Add To Favorites
This image is provided for informative purposes only and does not represent an actual product label. It should not be used as a substitute for official product labeling.

 

λmax 0.21-0.37 at 597-603 nm
application(s) diagnostic assay manufacturing
hematology
histology
color dark blue
concentration 2.5% in 2% acetic acid
form liquid
InChI 1S/C32H27N3/c1-23-22-26(16-21-31(23)33)32(24-12-17-29(18-13-24)34-27-8-4-2-5-9-27)25-14-19-30(20-15-25)35-28-10-6-3-7-11-28/h2-22,34H,33H2,1H3
InChI key LBLFVSANTXZPEX-UHFFFAOYSA-N
Quality Level 200 
SMILES string N(c2ccc(cc2)C(=C4C=CC(=Nc5ccccc5)C=C4)c3cc(c(cc3)N)C)c1ccccc1
solubility water: soluble
storage temp. room temp
technique(s) microbe id | staining: suitable
Application: Aniline blue (also known as cotton blue and ink blue) is a component of commonly used polychrome stains. It is used to stain collagen fibers in tissue sections using Masson′s trichrome protocol for staining multiple components. Collagen is stained blue by this method. The dye is suitable for selective staining of callose in plant specimens and staining histones for assessing nuclear maturity. Heidenhain′s AZAN stain and the Mallory′s polychrome stain both entail staining steps using aniline blue-orange G mixtures. Aniline blue is used in Gomori′s one-step trichrome stain and Mallory′s connective tissue stain for tissue including kidney and intestine.
General description: Aniline Blue is the traditional name applied to anionic dyes produced by successive phenylation and sulfonation of basic fuchsine. Aniline blue consists of sulfonation products of variable mixtures of phenylated rosaniline and pararosaniline. It is a mixture of methyl blue and water blue. The dye is also known as cotton blue, and ink blue. Methyl blue of aniline blue belongs to the group of triaminotriphenylmethane dyes.
RIDADR NONH for all modes of transport
WGK Germany WGK 2
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. room temp
UNSPSC 41116121

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