Alcian Blue solution
SIGMA/B8438 - 1% in 3% acetic acid, pH 2.5
Synonym: Alcian Blue 8XG solution
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: B8438-500ML.
| Amax | 0.45-0.60 (1:400 in H2O) |
| application(s) | diagnostic assay manufacturing hematology histology |
| color | dark blue to very dark blue |
| concentration | 1% in 3% acetic acid, pH 2.5 |
| form | liquid |
| pH range | 2.4-2.6 |
| Quality Level | 100  |
| storage temp. | room temp |
| technique(s) | microbe id | staining: suitable |
| Application: | Alcian Blue solution has been used in the histological staining of acidic glycosaminoglycans (GAGs; mucopolysaccharides) that are carboxylated or sulfated. Sulfated glycosaminoglycans appear to be the preferred substrate for alcian blue at pH 2.5. Alcian blue has been used to stain hyaluronan (HA), a nonsulfated GAG, but the intensity of the staining of HA is affected by the method of tissue fixation used. Alcian blue staining of GAGs in tissue may be modified through the addition of an electrolyte such as magnesium chloride. |
| Biochem/physiol Actions: | Alcian Blue is a cationic dye. It forms insoluble complexes with acidic glycosaminoglycans, thereby aiding in the quantitative determination of glycosaminoglycans. It is also used for the staining of bone and cartilage. |
| Packaging: | 250, 500 mL in glass bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 12171500 |