Monoclonal Anti-Bovine IgG2 antibody produced in mouse
SIGMA/B8400 - clone BG2-7, ascites fluid
MDL Number: MFCD00164545
Product Type: Chemical
ELISA Monoclonal Anti-Bovine IgG2 antibody produced in Mouse, Clone: BG2-7 (Cat. No. B8400), followed by 1:500 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917), was serially diluted to detect Bovine IgG2 by indirect ELISA. Reactivity is observed with: Bovine IgG and Bovine IgG2 but not with: Bovine IgG1, Bovine IgM and Human IgG.
| antibody form | ascites fluid |
| antibody product type | secondary antibodies |
| biological source | mouse |
| clone | BG2-7, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| dot blot: suitable | |
| indirect ELISA: 1:1,000 | |
| western blot: suitable |
| Application: | Monoclonal Anti-Bovine IgG2 antibody has been used in: • western blot • enzyme linked immunosorbent assay (ELISA) • dot blot assay |
| Biochem/physiol Actions: | Bovine IgGs are glycoprotein antibodies that regulate immune responses in herds. These antibodies inhibit TLR5 activation upon immunization with native H7 flagellin. Bovine IgG levels can be used for the detection of Johne′s disease and milk adulteration. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The IgG class antibodies contain two subclasses, IgG1 and IgG2, which have antigenic differences in the Fc portion of the heavy chains. Monoclonal Anti-Bovine IgG2 (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes of an immunized mouse. |
| Immunogen: | Purified bovine IgG |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |