Anti-Bovine IgG (whole molecule) antibody produced in rabbit
SIGMA/B8395 - whole antiserum
MDL Number: MFCD00162167
Product Type: Chemical
ELISA Anti-Bovine IgG (whole molecule) antibody produced in Rabbit (Cat. No. B8395), followed by 1:5,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545), was serially diluted to detect Bovine IgG by indirect ELISA.
| antibody form | whole antiserum |
| antibody product type | secondary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 1:150,000 |
| quantitative precipitin assay: 4.0 mg/mL |
| Application: | Anti-Bovine IgG (whole molecule) antibody produced in rabbit has been used as a standard to determine protein content by Bradford method using Bio-Rad protein assay dye reagent. It has also been used in several assays to develop a particle concentration fluorescence immunoassay for the quantitative determination of IgG in bovine milk. |
| Application: | Anti-Bovine IgG (whole molecule) antibody produced in rabbit has been used for detecting IgG antibodies in multichannel monolithic quartz crystal microbalance (MQCM). |
| Biochem/physiol Actions: | IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. |
| Biochem/physiol Actions: | Immunoglobulins G (IgGi, IgGa) of bovine milk is capable of protecting the gut mucosa against microorganisms. |
| Biochem/physiol Actions: | The antiserum is determined to be immunospecific for bovine IgG. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Physical form: | Rabbit Anti-Bovine IgG is supplied as a liquid containing 0.1% sodium azide (see MSDS) as a preservative |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| Preparation Note: | treated to remove lipoproteins |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |