Biglycan from bovine articular cartilage
SIGMA/B8041 - essentially salt-free, lyophilized powder
MDL Number: MFCD00676525
Product Type: Chemical
| biological source | bovine articular cartilage |
| form | essentially salt-free, lyophilized powder |
| mol wt | 200-350 kDa |
| packaging | glass bottle of 0.5 mg |
| Quality Level | 200  |
| shipped in | dry ice |
| solubility | water: soluble ≥2.00 mg/mL, clear, colorless |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P21809  |
| Biochem/physiol Actions: | Biglycan interacts with collagen type I and II, fibronectin and TGF-ß. In a low ionic strength (less than 3 mM phosphate) buffer, Biglycan binds more strongly to collagen type I. At higher ionic strengths, Biglycan will instead increase the inhibition effect of TGF-ß on osteoclast proliferation at a concentration of 4-20 mg/mL. It also functions to prevent the attachment of CHO cells to fibronectin, with a 50% inhibition at 17-21 mg/mL. As an extracellular component, Biglycan binds TGF-β 1, facilitates bone mineralization and supports osteoblast differentiation thru BMP-4 binding. |
| Disclaimer: | Store this product at -20°C. Stored as supplied, this powder shows little decomposition in 3 years when stored properly. |
| General description: | proteoglycan consisting of a 45 kDa core protein and two chrondroitin/dermatan sulfate glycosaminoglycan chains |
| Other Notes: | Biglycan is a 200-350 kDa proteoglycan consisting of a 45 kDa core protein and two chondroitin/dermatan sulfate glycosaminoglycan chains. |
| Packaging: | 0.5 mg in glass bottle |
| Preparation Note: | This product is extracted from articular cartilage, chematographically purified, dialyzed against water, and 0.2 μm filtered prior to lyophilization. Once lyophilized, this powder is essentially salt-free. The product is soluble in water at 2 mg/mL. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |