CelLytic™ B Cell Lysis Reagent
SIGMA/B7435 - For bacterial cell lysis, standard strength
Synonym: Cell lysis reagent
MDL Number: MFCD02097909
Product Type: Chemical
| form | solution |
| Quality Level | 200 ![]() |
| Application: | A proprietary, non-denaturing formulation of zwitterionic detergents used for the lysis of bacterial cells and extraction of recombinant proteins. |
| Application: | CelLytic™ B Cell Lysis Reagent has been used for cell lysis for the extraction of coenzyme Q10, recombinant Src kinase associated phosphoprotein 1- glutathione S-transferases (GST) fusion protein and human α-synuclein. |
| Features and Benefits: | • Higher protein extraction efficiency than traditional methods such as sonication and lysozyme • Scalable for 1 to 25 grams of bacterial cell paste • No interference with downstream applications such as affinity chromatography, IP, and Western blotting • Compatible with protease inhibitors, inhibitor cocktails, chaotropes, salts, chelating agents and reducing agents |
| Legal Information: | CelLytic is a trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | Covered by US Patent No 7,282,475 B2 and are sold for research use only. Commercial use requires addtional licenses. |
| Legal Information: | Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Detergent blend formulated in 40 mM Trizma® HCl (pH 8.0). |
| Symbol | GHS07 |
| Signal word | Warning |
| Hazard statements | H315 - H319 |
| Precautionary statements | P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41116134 |


