Anti-Digoxin−Biotin antibody, Mouse monoclonal
SIGMA/B7405 - clone DI-22, purified from hybridoma cell culture
Synonym: Monoclonal Anti-Digoxin; Monoclonal Anti-Digoxin−Biotin antibody produced in mouse
Product Type: Chemical
ELISA Monoclonal Anti-Digoxin-Biotin antibody produced in Mouse, Clone: DI-22 (Cat. No. B7405) followed by 2 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886) was serially diluted to detect Digoxin by indirect ELISA. Reactivity is observed with Digoxin-BSA and Digoxin-Transferrin but not with Transferrin and BSA.
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | DI-22, monoclonal |
| conjugate | biotin conjugate |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 1:10,000 using digoxin-labeled biomolecule |
| western blot: 1:500 using using digoxin-labeled biomolecule |
| Application: | Monoclonal Anti-Digoxin-Biotin antibody produced in mouse has been used in: • in situ hybridization • immunohistochemistry • immunolocalization |
| Application: | Monoclonal Anti-Digoxin-Biotin antibody produced in mouse may be used for ELISA at a dilution of 1:10,000 and for dot blots at a dilution of 1:500. It was also used to stain apoptotic cells in cervical carcinoma sections by TUNEL technique. |
| Biochem/physiol Actions: | Antibody is specific for digoxin and shows a high affinity for digoxigenin. |
| Biochem/physiol Actions: | Digoxin is a phytoestrogen that binds estrogen receptors and produces inotropic effects in cardiac muscle. Studies show that digoxin decreases the risk of breast and uterus cancers that are estrogen sensitive. |
| Biochem/physiol Actions: | The conjugate may be used to detect digoxin-labeled compounds such as oligonucleotides, antibodies or peptides. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Digoxin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. |
| Immunogen: | Digoxin-KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |