Anti-Breast Cancer Resistance Protein antibody produced in rabbit
SIGMA/B7185 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-BCRP
MDL Number: MFCD03791618
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human placenta tissue sections were stained with 20 μg/mL Anti-Breast Cancer Resistance Protein antibody produced in Rabbit (Cat. No. B7185) followed by 20 μg/mL Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat (Cat. No. B8895) and by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886).
Immunoblotting Human full-term placenta cell extract was separated on SDS-PAGE and probed with AIA To Breast Cancer Resistance Protein (BCRP) (RB) (Cat. No. B7185). The antibody was developed with HRP Anti-Rabbit IgG (Jackson’s Cat. No. 111-035-045) and ECL substrate. Antibody dilution 2.5 μg/mL.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~70 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20-40 μg/mL using heat-retrieved tissue sections from human term placenta by indirect immunoperoxidase staining of syncytiotrophoblasts. |
| western blot: 2.5-5 μg/mL using whole extract of human term placenta or mouse kidney and a chemiluminescent detection reagent | |
| UniProt accession no. | Q9UNQ0  |
| Application: | Anti-Breast Cancer Resistance Protein antibody produced in rabbit has been used in: • western blotting • immunocytochemistry • immunohistochemistry |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Biochem/physiol Actions: | Up regulation of breast cancer resistance protein (BCRP) expression may be found in vivo and in vitro in both drugs treated and untreated tumors of different types. Breast cancer resistance protein transports various xenobiotics to the extracellular space through a process energized by ATP hydrolysis. In addition, to its role in protecting cells and tissues against xenobiotics especially in the intestine, liver, placenta and the blood-brain barrier, BCRP appears to be involved in hypoxic defense mechanisms and the protection and regulation of stem cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Breast Cancer Resistance Protein is developed in rabbit using as immunogen a synthetic peptide of human breast cancer resistance protein (BCRP). BCRP, also designated ABCG2, ABCP, placenta specific ATP-binding transporter and mitoxantrone-resistance protein (MXR), is a member of the ABCG subfamily of the large ATP binding cassette (ABC) transporter family of transmembrane proteins. BCRP is a half-transporter, with a single ATP binding cassette and transmembrane region. BCRP is normally expressed in human placental syncytiotrophoblasts, mammary ducts and lobules, endothelial cells and stem cells of different tissues. |
| Immunogen: | synthetic peptide corresponding to amino acid residues 150-167 of human breast cancer resistance protein with C-terminal added cysteine, conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |