Anti-Goat IgG (whole molecule)–Biotin antibody produced in rabbit
SIGMA/B7014 - affinity isolated antibody, buffered aqueous solution
MDL Number: MFCD00162346
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human heart were stained with 20 μg/mL Anti-Cytochrome c antibody produced in Sheep (Cat. No. C9616), followed by 0.4 μg/mL Anti-Goat IgG (whole molecule)-Biotin antibody produced in Rabbit (Cat. No. B7014) and by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886).
ELISA Anti-Goat IgG (whole molecule)-Biotin antibody produced in Rabbit (Cat. No. B7014), followed by 2 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886), was serially diluted to detect Goat IgG by direct ELISA.
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | biotin conjugate |
| form | buffered aqueous solution |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:20,000 |
| Application: | Anti-Goat IgG (whole molecule)-Biotin antibody produced in rabbit has been used: • immunohistochemistry • immunostaining • western blot • immunofluorescence • immunohistochemical studies |
| Biochem/physiol Actions: | Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. It mainly helps in immune defense. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | An immunoglobulin has two heavy chain and two light chain connected by disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Goat IgG has two subclasses- IgG1 and IgG2. |
| General description: | Goat IgG is often used for the detection and study of various proteins such as EPAS1 and MIP-3 α among several others. Anti-goat IgG (whole molecule)-Biotin antibody can be used as a secondary antibody to facilitate the detection of such target proteins bound to goat IgGs by immunological assays . Thus, anti-goat IgGs are useful tools for the analysis of target proteins. Anti-Goat IgG (whole molecule)-Biotin antibody is specific for IgG in goats. |
| Immunogen: | Goat IgG |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |