Bradford Reagent
SIGMA/B6916 - for 0.1-1.4 mg/ml protein
Synonym: Coomassie dye binding protein assay; Coomassie dye binding protein assay, Protein dye reagent; Protein dye reagent
Product Type: Chemical
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| form | solution |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| Application: | Bradford Reagent has been used to determine total protein concentration. |
| Features and Benefits: | • The reagent is ready to use. No mixing or dilution required. • Color development is rapid. Only a five minute incubation and then the sample is read a 595 nm. • Reducing sugars and reducing substances along with thiols do not interfere with this reagent. • Reagent is suitable for micro (1-10 μg/ml) and standard (50-1400 μg/ml) assays. • Can be used in microwell plate assays. • Inexpensive assay. |
| General description: | Bradford assay is addition of coomassie brilliant blue G-250 to protein solution. The coomassie blue dye associates with basic and aromatic amino acids, thereby causing shift in absorbance during protein determination. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research  |
| Symbol |   GHS05,GHS08 |
| Signal word | Warning |
| Hazard statements | H290 - H315 - H319 - H371 |
| Precautionary statements | P234 - P260 - P264 - P302 + P352 - P305 + P351 + P338 - P308 + P311 |
| Hazard Codes | Xn |
| Risk Statements | 20/21/22-36/38-68/20/21/22 |
| Safety Statements | 26-36/37 |
| RIDADR | UN 1805 8 / PGIII |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12161500 |