BIS-TRIS propane

SIGMA/B6755 - ≥99.0% (titration)

Synonym: 1,3-Bis[tris(hydroxymethyl)methylamino]propane; 2,2′-(Propane-1,3-diylbis(azanediyl))bis(2-(hydroxymethyl)propane-1,3-diol); 1,3-Bis[tris(hydroxymethyl)methylamino]propane

CAS Number: 64431-96-5
Empirical Formula (Hill Notation): C₁₁H₂₆N₂O₆
Molecular Weight: 282.33
EC Number: 264-899-3
MDL Number: MFCD00004689
Linear Formula: CH₂[CH₂NHC(CH₂OH)₃]₂
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-B6755-25G	25 g	$73.70 1/EA	Add To Favorites
45-B6755-100G	100 g	$265.00 1/EA	Add To Favorites
45-B6755-250G	250 g	$495.00 1/EA	Add To Favorites
45-B6755-500G	500 g	$846.00 1/EA	Add To Favorites
45-B6755-1KG	1 kg	$1480.00 1/EA	Add To Favorites
45-B6755-10KG	10 kg	No Price

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This image depicts SKU: B6755-100G

Properties
Descriptions
Safety Info.
Basic Data

application(s)	diagnostic assay manufacturing general analytical life science and biopharma
assay	≥99.0% (titration)
color	white
density	1.179 g/cm³ at 20—25 °C
	1.189 g/cm³ at 20—25 °C
form	powder
InChI	1S/C11H26N2O6/c14-4-10(5-15,6-16)12-2-1-3-13-11(7-17,8-18)9-19/h12-19H,1-9H2
InChI key	HHKZCCWKTZRCCL-UHFFFAOYSA-N
mp	164-165 °C (lit.)
pKa (25 °C)	(1) 6.8, (2) 9.0
Quality Level	400
SMILES string	OCC(CO)(CO)NCCCNC(CO)(CO)CO
solubility	water: 30 % (w/w), clear, colorless
storage condition	dry at room temperature
storage temp.	room temp
suitability	suitable for chromatography
	suitable for electrophoresis buffers
technique(s)	HPLC: suitable
useful pH range	6.3-9.5

Application:	BIS-TRIS Propane may be used in the following studies:• as a buffer to investigate the influence of anions with varying nucleophilic properties on the autoxidation of oxymyoglobin (MbO₂). • for purification of glucose-binding protein from membranes of Sulfolobus solfataricus. • as a buffer during GeO₂mineralisation. • as a buffer component in a study pertaining to environmental research • as a buffer component to maintain optimal pH conditions during the purification and preparation of engineered enzymes
Features and Benefits:	• Suitable for Biological and Biochemical Research • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications • Effective Buffering from pH 6.3-9.5 (25 °C) with a pKa of 6.8 and 9.0 (25 °C) • Highly soluble in water
General description:	Bis-Tris Propane buffer, also known as BTP, is a zwitterionic biochemical buffer utilized for pH regulation in various laboratory applications, particularly in molecular biology, biochemistry, and electrophoresis. Its wide buffering range, spanning from pH 6.3 to 9.5, is attributed to its two pKa values of 6.8 (pKa1) and 9.0 (pKa2), which are close. This wide range makes it highly versatile for maintaining stable pH conditions in a variety of experiments. Furthermore, Bis-Tris Propane is a water-soluble buffer substance that plays a crucial role in enhancing the stability and activity of restriction enzymes. Specifically, its buffering capacity is advantageous for experiments that require pH levels as low as 6-7. When compared to Tris buffer, which has a poor buffering capacity below pH 7.5 and exhibits significant pKa fluctuations with temperature changes, Bis-Tris Propane proves to be a superior choice. Additionally, it finds application in conjunction with hydrochloric acid buffer to stabilize farnesyl diphosphate, particularly when isolated from Saccharomyces cerevisiae strains. Notably, it can also serve as a ligand, forming dinuclear hydroxo complexes with Lanthanides(III).
Other Notes:	For additional information on our range of Biochemicals , please complete this form .
Packaging:	1 kg in poly bottle
Packaging:	25, 100, 250, 500 g in poly bottle

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥99.0% (titration)
mp	164-165 °C (lit.)
Density	1.179 g/cm³ at 20—25 °C; 1.189 g/cm³ at 20—25 °C
Storage Temp.	room temp
UNSPSC	12161700

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