Casein Blocking Buffer 10x
SIGMA/B6429 - for Northern and Southern blotting, solution
Synonym: 10x Blocking Buffer
Product Type: Chemical
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| form | solution |
| grade | Molecular Biology |
| Quality Level | 200  |
| storage temp. | room temp |
| technique(s) | Southern blotting: suitable |
| Application: | Casein Blocking Buffer 10x has been used in: • western blotting • enzyme linked immunosorbent assay (ELISA) • immunohistochemistry |
| Features and Benefits: | • Better blocking performance than dried milk or BSA • Easy-to-use concentrate • Compatible with neutral and positively-charged membranes • Compatible with chemiluminescent and colorimetric detection assays |
| General description: | Casein is a phosphoprotein found in milk. It is classified into four subtypes, including αS1-, αS2-, β-, and κ-caseins. Sigma’s 10x Blocking Buffer is a casein-based blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. |
| Preparation Note: | Dilute 10x Blocking Buffer with molecular biology grade water before use. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 12161700 |