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BCIP®/NBT solution, premixed

SIGMA/B6404

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-B6404-100ML 100 mL
 $98.40
1/EA		 Add To Favorites
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo-4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures.This substrate system produces an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually. Immunohistochemical staining with BCIP/NBT utilizes requires organic mounting media and can be counterstained with Nuclear Fast Red or Light Green.

 

form liquid
Quality Level 100 
Application: BCIP/NBT solution, premixed has been used:
• as a substrate to develop dot-blots
• as a substrate to reveal alkaline phosphatase in the immunohistochemical analysis
• to detect specific immune complexes in western blot analysis
General description: Substrate for visualizing alkaline phosphatase conjugates in Western blotting.
Legal Information: BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes: 0.48 mM NBT, 0.56 mM BCIP, 10 mM Tris and 59.3 mM MgCl2, pH approx. 9.2
RIDADR NONH for all modes of transport
WGK Germany WGK 1
Flash Point(F) Not applicable
Flash Point(C) Not applicable
UNSPSC 41105333

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