5-Bromo-4-chloro-3-indolyl phosphate disodium salt

SIGMA/B6149 - ≥98% (HPLC)

Synonym: 5-BCIP disodium salt; BCIP^®; X-phosphate disodium salt

CAS Number: 102185-33-1
Empirical Formula (Hill Notation): C₈H₄BrClNNa₂O₄P
Molecular Weight: 370.43
MDL Number: MFCD00036757
Linear Formula: C₈H₄BrClNO₄P · 2Na
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-B6149-25MG	25 mg	$73.00 1/EA		Add To Favorites
45-B6149-50MG	50 mg	$78.40 1/EA		Add To Favorites
45-B6149-100MG	100 mg	$118.00 1/EA		Add To Favorites
45-B6149-500MG	500 mg	$389.00 1/EA		Add To Favorites
45-B6149-1G	1 g	$588.00 1/EA		Add To Favorites
45-B6149-5G	5 g	$2380.00 1/EA		Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

assay	≥98% (HPLC)
form	powder
InChI	1S/C8H6BrClNO4P.2Na/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;;/h1-3,11H,(H2,12,13,14);;/q;2*+1/p-2
InChI key	OAZUOCJOEUNDEK-UHFFFAOYSA-L
Quality Level	200
SMILES string	[Na+].[Na+].[O-]P([O-])(=O)Oc1c[nH]c2ccc(Br)c(Cl)c12
solubility	DMF: insoluble
	H₂O: 20 mg/mL
storage temp.	−20°C

Application:	5-Bromo-4-chloro-3-indolyl phosphate disodium salt has been used to detect protein-antibody complexes. It has also been used for tissue staining in alkaline phosphatase histochemistry.
Application:	For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
Application:	For the colorimetric detection of alkaline phosphatase-labeled molecules.
General description:	Histochemical substrate for alkaline phosphatase.
Legal Information:	BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
Packaging:	1, 5 g in poly bottle
Packaging:	25, 50, 100 mg in poly bottle
Packaging:	500 mg in poly tube

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥98% (HPLC)
Storage Temp.	−20°C
UNSPSC	12352204

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