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BIS-TRIS hydrochloride

SIGMA/B6032 - ≥99.0% (titration)

Synonym: 2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol; 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol; Bis(2-hydroxyethyl)aminotris(hydroxymethyl)methane

CAS Number: 124763-51-5
Empirical Formula (Hill Notation): C8H19NO5 · HCl
Molecular Weight: 245.70
MDL Number: MFCD00171252
Linear Formula: C8H19NO5 · HCl
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-B6032-25G 25 g
 $90.90
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45-B6032-100G 100 g
 $302.00
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45-B6032-500G 500 g
 $851.00
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45-B6032-1KG 1 kg
 $1500.00
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This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.

This image depicts SKU: B6032-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.

This image depicts SKU: B6032-25G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.

This image depicts SKU: B6032-500G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.

This image depicts SKU: B6032-1KG

 

application(s) diagnostic assay manufacturing
assay ≥99.0% (titration)
form crystalline powder
InChI 1S/C8H19NO5.ClH/c10-3-1-9(2-4-11)8(5-12,6-13)7-14;/h10-14H,1-7H2;1H
InChI key VEYRVLHAMHQVTC-UHFFFAOYSA-N
pKa  6.5
Quality Level 200 
SMILES string Cl.OCCN(CCO)C(CO)(CO)CO
solubility water: 0.5 g/mL, clear, colorless to very faintly yellow
storage temp. room temp
useful pH range 5.8-7.2
Application:

  • Phos-tag SDS-PAGE systems for phosphorylation profiling of proteins with a wide range of molecular masses under neutral pH conditions.: This research introduces the use of BIS-TRIS hydrochloride in Phos-tag SDS-PAGE systems to profile protein phosphorylation. The system operates under neutral pH conditions, making it suitable for analyzing proteins with diverse molecular weights. This method enhances the resolution of phosphorylated proteins, facilitating detailed phosphorylation studies crucial for understanding cellular signaling and protein function (Kinoshita et al., 2012 ).
Packaging: 1 kg in poly bottle
Packaging: 25, 100, 500 g in poly bottle
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥99.0% (titration)
Storage Temp. room temp
UNSPSC 12161700

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