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5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside

SIGMA/B6024 - tablet

Synonym: 5-Bromo-4-chloro-3-indolyl β-D-galactoside; BCIG; X-Gal

CAS Number: 7240-90-6
Empirical Formula (Hill Notation): C14H15BrClNO6
Molecular Weight: 408.63
EC Number: 230-640-8
MDL Number: MFCD00005666
Linear Formula: C14H15BrClNO6
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-B6024-10TAB 10 tabs
 $297.00
1/EA		 Add To Favorites
45-B6024-50TAB 50 tabs
 $825.00
1/EA		 Add To Favorites
FT-IR Spectra for 5-Bromo-4-chloro-3-indolyl β-ᴅ-galactoside.

 

form tablet
InChI 1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1
InChI key OPIFSICVWOWJMJ-AEOCFKNESA-N
Quality Level 200 
SMILES string OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O
storage temp. −20°C
suitability suitable for identification of lac+ bacterial colonies
Application: 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside has been used in the preparation of X-Gal stock solution to add it in LB agar plates for blue-white screening of transformants.
Application: X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.
Biochem/physiol Actions: X-gal is routinely used to detect transgene activity in situ.
General description: 5-Bromo-4-chloro-3-indolyl β -D-galactopyranoside, commonly known as X-Gal, is a histochemical substrate for β-galactosidase.
Preparation Note: A stock solution (100 mg/ml) is prepared by dissolving X-Gal in dimethylformamide and should be stored at −20°C and protected from light. Sterilization is not required.
Preparation Note: Contains 5 mg substrate per tablet
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. −20°C
UNSPSC 12352204

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