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Bromophenol Blue sodium salt

SIGMA/B5525

Synonym: 3′,3′′,5′,5′′-Tetrabromophenolsulfophthalein sodium salt

CAS Number: 34725-61-6
Empirical Formula (Hill Notation): C19H9Br4NaO5S
Molecular Weight: 691.94
EC Number: 252-170-2
MDL Number: MFCD00013793
Linear Formula: C19H9Br4NaO5S
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-B5525-5G 5 g
 $42.10
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45-B5525-10G 10 g
 $59.80
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45-B5525-25G 25 g
 $151.00
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This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: B5525-25G

 

form powder
InChI 1S/C19H10Br4O5S.Na/c20-12-5-9(6-13(21)18(12)24)17(10-7-14(22)19(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28;/h1-8,24H,(H,26,27,28);/q;+1/p-1
InChI key TWKDJMRHSLWRFI-UHFFFAOYSA-M
Quality Level 200 
SMILES string [Na+].Oc1c(Br)cc(cc1Br)C(=C2/C=C(Br)C(=O)C(Br)=C2)c3ccccc3S([O-])(=O)=O
solubility water: soluble
storage temp. room temp
suitability suitable for nucleic acid electrophoresis
technique(s) electrophoresis: suitable
Application: A tracking dye for nucleic acid or protein electrophoresis in agarose or polyacrylamide gels.
Application: Bromophenol blue sodium salt has been used in the preparation of protein samples for western blotting analysis. It has also been used in Drosphila fly food to aid visualization of gut for dissection.
General description: Bromophenol Blue is a pH indicator, and a dye appearing as a strong blue color. It is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. Bromophenol blue has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.
Packaging: 5, 10, 25 g in glass bottle
Preparation Note: A 6x Gel Loading Buffer can be made using standard recipes, including 0.015% bromophenol blue.
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. room temp
UNSPSC 12171500

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