Brilliant Cresyl Blue ALD
SIGMA/B5388 - certified by the BSC
Synonym: 3-
CAS Number: 51716-96-2
Empirical Formula (Hill Notation): C17H20ClN3O · 0.5 ZnCl2
Molecular Weight: 385.96
EC Number: 279-675-0
MDL Number: MFCD00148901
Linear Formula: C17H20N3OCl · 1/2ZnCl2
Product Type: Chemical
| agency | certified by the BSC |
| application(s) | diagnostic assay manufacturing hematology histology |
| composition | Dye content, ≥60% |
| form | powder |
| InChI | 1S/2C17H20N3O.4ClH.Zn/c2* |
| InChI key | SIHASHFMDFXGSX-UHFFFAOYSA |
| Quality Level | 200 ![]() |
| SMILES string | [Zn-2](Cl)(Cl)(Cl)Cl.N(CC |
| solubility | water: 1 mg/mL, blue to very deep blue |
| storage temp. | room temp |
| Application: | Brilliant Cresyl Blue ALD has been used for the selection of oocytes. It has also been used for the selection of cumulus-oocyte complexes (COCs). |
| Application: | Certified as a supravital stain for reticulocytes. |
| Biochem/physiol Actions: | Brilliant Cresyl Blue ALD is mainly used for the selection of oocytes. It helps in determining the activity of glucose-6-phosphate dehydrogenase (G6PD). This enzyme is generated in growing oocytes. However, oocytes which complete their growth have low levels of G6PD. Low G6PD activity results in blue coloration of the cytoplasm because brilliant cresyl blue is not reduced to a colourless compound. It is also used for the staining in reticulocytes, where the dye stains reticulin. |
| Other Notes: | The structure of Brilliant Cresyl Blue ALD contains a 2-methyl modification on the phenoxazine core, while the structure of the historic Brilliant Cresyl Blue contains a 8-methyl modification on the phenoxazine core. The biological staining application is the same for both structures. |
| Packaging: | 10 g in glass bottle |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26-36 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| Colour Index Number | 51010 |
| UNSPSC | 12171500 |

