Anti-Rabbit IgG (γ-chain specific)−Biotin antibody, Mouse monoclonal
SIGMA/B5283 - clone RG-96, purified from hybridoma cell culture
Synonym: Monoclonal Anti-Rabbit IgG (γ-chain specific)
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded, Human tonsil tissue sections were stained with 1:100 Anti-Human IgG (whole molecule) antibody produced in Rabbit (Cat. No. I8635), followed by 1:1,000 Anti-Rabbit IgG (γ-chain specific)-Biotin antibody, Mouse monoclonal, Clone: RG-96 (Cat. No. B5283) and by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886).
Western Blotting Whole extract of Human HeLa cells were separated on SDS-PAGE and probed with 2 μg/mL Anti-Actin, N-terminal antibody produced in Rabbit (Cat. No. A2103). The antibody was developed using Anti-Rabbit IgG (γ-chain specific)-Biotin antibody, Mouse monoclonal (Cat. No. B5283) followed by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886). Lanes 1. 1:200,000 antibody 2. 1:400,000 antibody 3. Negative control
ELISA Anti-Rabbit IgG (γ-chain specific)-Biotin antibody, Mouse monoclonal (Cat. No. B5283), followed by 2 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886), was serially diluted to detect Rabbit IgG by indirect ELISA.
| antibody form | purified immunoglobulin |
| antibody product type | secondary antibodies |
| biological source | mouse |
| clone | RG-96, monoclonal |
| conjugate | biotin conjugate |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| should not react with | goat, feline, pig, guinea pig, rat, bovine, canine, human, horse, sheep, chicken |
| species reactivity | rabbit |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:150,000 |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 | |
| western blot (chemiluminescent): 1:200,000-1:400,000 |
| Application: | Monoclonal Anti-Rabbit IgG (γ-chain specific)-Biotin is suitable for ELISA at a working dilution of 1:150,000. It may be used for immunohistochemistry of formalin-fixed, paraffin-embedded sections at a dilution of 1:1000. The antibody was used in β-catenin ELISA at a dilution of 1:2000. Anti-Rabbit IgG (γ-chain specific)-Biotin antibody, Mouse monoclonal has been used in β-Catenin enzyme immunoassay. It has also been used in immunohistochemistry for examining histology of mammary glands. |
| Biochem/physiol Actions: | IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin to monoclonal Anti-Rabbit IgG (γ-chain specific) antibody allows for the binding of various labels such as avidin or streptavidin. IgG antibody has its function similar to IgM antibody in complement system activation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | IgG (immunoglobulin G) antibody is secreted by B cells and is found in blood and extracellular fluids and is enriched in mother’s milk. The variable region of IgG antibody is specific to antigens and is highly conserved. |
| General description: | The product recognizes an epitope located on the γ (heavy)-chain of rabbit IgG. In immunoblotting, the antibody recognizes both native and denatured forms of rabbit IgG. In ELISA, the antibody is specific for rabbit IgG, and shows no cross-reactivity with rabbit IgA and IgM or human IgG, IgA, and IgM. No cross reaction is observed with IgG from the following species: bovine, cat, chicken, dog, goat, guinea pig, horse, pig, rat, or sheep. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |