Bromelain from pineapple stem
SIGMA/B5144 - ≥4 units/mg protein, (chromatography purified)
CAS Number: 37189-34-7
EC Number: 253-387-5
MDL Number: MFCD00081423
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: B5144-100UN
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: B5144-250UN
| composition | Protein, 30-50% biuret |
| form | lyophilized powder |
| Quality Level | 200  |
| solubility | H2O: soluble 1.0 mg/mL, clear, colorless |
| specific activity | (chromatography purified) |
| ≥4 units/mg protein | |
| storage temp. | −20°C |
| Application: | Bromelain from pineapple stem has been used as a positive control in sodium dodecyl sulfate (SDS) / polyacrylamide gel electrophoresis (PAGE) zymograms. It has also been used as a Azocasein substrate for the determination of proteolytic activity. |
| Application: | Bromelain may be used to inhibit the biosysnthesis of proinflammatory prostaglandins. It may be used to reduce clotting efficiency. Bromelain, from pineapple stem, has been used to make enzymatic hydrolysates of honeybee-collected pollen . |
| Biochem/physiol Actions: | Bromelain is a cysteine endopeptidase with broad specificity for cleavage of proteins. Bromelain may be from a stem or piece of fruit. Product P3169 (pGlu-Phe-Leu-p-nitroanil |
| Other Notes: | One unit will release 1.0 μmole of p-nitrophenol from N-α-Z- |
| Packaging: | 100, 250 units in glass bottle |
| Physical form: | Lyophilized powder containing mannitol and potassium phosphate buffer salts |
| Preparation Note: | Chromatographically purified |
| Preparation Note: | Chromatographically purified. Dissolves in water at 1 mg/mL concentration to form a clear, colorless solution. |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H334 - H335 |
| Precautionary statements | P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 36/37/38-42 |
| Safety Statements | 22-24-26-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: (chromatography purified); specific activity: ≥4 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.22.32 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |