Butyrylcholinesterase human
SIGMA/B4186 - vial of ≥4 units
Synonym: Acylcholine acyl-hydrolase; Choline esterase, butyryl; Pseudocholinesterase
CAS Number: 9001-08-5
EC Number: 232-579-2
MDL Number: MFCD00081455
Product Type: Chemical
Common enzymatic methods for measuring organophosphate and cholinesterase activity include: colorimetric (using DTNB or ferricyanate to measure thiolesterase products), colorimetric (coupled with choline oxidase and peroxidase), fluorescent, titrimetric (mainly for purified enzyme activity assays). Shown above is the simple uncoupled assay using acetylcholine which can be measured titrimetrically.
Common enzymatic methods for measuring organophosphate and cholinesterase activity include: colorimetric (using DTNB or ferricyanate to measure thiolesterase products), colorimetric (coupled with choline oxidase and peroxidase), fluorescent, titrimetric (mainly for purified enzyme activity assays). Shown above is the coupled assay using choline oxidase and peroxidase.
Common enzymatic methods for measuring organophosphate and cholinesterase activity include: colorimetric (using DTNB or ferricyanate to measure thiolesterase products), colorimetric (coupled with choline oxidase and peroxidase), fluorescent, titrimetric (mainly for purified enzyme activity assays). Shown above is the thiolesterase assay using DTNB detection.
Common enzymatic methods for measuring organophosphate and cholinesterase activity include: colorimetric (using DTNB or ferricyanate to measure thiolesterase products), colorimetric (coupled with choline oxidase and peroxidase), fluorescent, titrimetric (mainly for purified enzyme activity assays). Shown above is the thiolesterase assay using ferricyanate detection.
| form | powder |
| packaging | vial of ≥4 units |
| Quality Level | 200  |
| specific activity | ≥50 units/mg protein |
| storage temp. | 2-8°C |
| UniProt accession no. | P06276  |
| Analysis Note: | Starting material individually donor tested and found negative for HIV I & II antibodies, Hepatitis B surfaceantigen, and Hepatitis C antibodies. |
| Application: | BchE, from Sigma, has been used to determine butyrylcholinesterase inhibition by various test compounds . |
| Application: | Butyrylcholinesterase human has been used to study its role as a modulator of pathological features of Alzheimer′s disease (AD) in vivo. |
| Biochem/physiol Actions: | Butyrylcholinesterase (BChE) is a serine hydrolase that shares substantial structural similarities with acetylcholinesterase (AchE) but has different substrate and inhibitor specificities. BChE is found in the serum, hemopoietic cells, liver, lung, heart and the central nervous system of vertebrates. |
| Biochem/physiol Actions: | Butyrylcholinesterase (BChE) is a serine hydrolase that shares substantial structural similarities with acetylcholinesterase (AchE) but has different substrate and inhibitor specificities. It is found in the serum, hemopoietic cells, liver, lung, heart and the central nervous system of vertebrates. BChE hydrolyzes large esters of choline such as butyrylcholine and benzoylcholine. Selective inhibition of BChE activity can be used in the detection of organophosphates. BchE may be used to treat organophosphate toxicity. It is a critical target for Alzheimer′s disease (AD) therapy. |
| Other Notes: | One unit will hydrolyze 1.0 μmole of butyrylcholine to choline and butyrate per min at pH 8.0 at 37 °C. The activity obtained using butyrylcholine as substrate is ~2.5 times that obtained using acetylcholine. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥50 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.1.1.8 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |