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BCIP®/NBT-Purple Liquid Substrate System for Membranes

SIGMA/B3679 - ready to use alkaline phosphatase substrate

Synonym: 5-bromo-4-chloro-3-indolyl-phosphate / Nitro blue tetrazolium chloride liquid substrate system

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-B3679-100ML 100 mL
 $274.00
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Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo-4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures.This substrate system produces an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually. Immunohistochemical staining with BCIP/NBT utilizes requires organic mounting media and can be counterstained with Nuclear Fast Red or Light Green.

 

form liquid
Quality Level 100 
storage temp. 2-8°C
Application: BCIP®/NBT-Purple Liquid Substrate System for Membranes has been used as a substrate for alkaline phosphatase
• for the detection of secondary antibodies by immunoblot
• in ELISPOT assay
• in dot blot analysis
• in western blot to visualize protein bands on nitrocellulose membrane
Biochem/physiol Actions: 5-bromo-4-chloro-3-indolyl phosphate (BCIP)/ nitro blue tetrazolium (NBT) is a chromogenic substrate system for the detection of alkaline phosphatase (AP) enzyme. AP hydrolyses BCIP to form an intermediate leucoindigo compound and is further oxidized by NBT to form 5,5′-dibromo-4,4′-dichloro indigo (BCI). NBT is reduced to an insoluble purple diformazan (DF) and forms NBT-DF. A mixture of NBT-DF and BCI results in the dark purple colored precipitate. AP by hydrolysing many BCIP molecules makes it a highly sensitive method.
Disclaimer: Stable at least one year at 2-8 °C.
Legal Information: BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
Physical form: Ready-to-use.
Storage Temp. 2-8°C
UNSPSC 12352204

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