Monoclonal Anti-Bovine Serum Albumin antibody produced in mouse
SIGMA/B2901 - clone BSA-33, ascites fluid
Synonym: Anti Bsa; Anti-Bsa Antibody; BSA Antibody - Monoclonal Anti-Bovine Serum Albumin antibody produced in mouse; Bsa Antibody; Anti-Albumin, bovine serum; Anti-BSA
MDL Number: MFCD00162166
Product Type: Chemical
Immunoprecipitation Monoclonal Anti-Bovine Serum Albumin, Clone: BSA-33 (Cat. No. B2901) was used to immunoprecipitate BSA from Fetal bovine serum (Cat. No. F7524). Detection antibody: Rabbit Anti-Bovine Albumin (Cat. No. B1520), followed by Anti-Rabbit IgG-peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Positive control: total 5 μg BSA without IP 2. Negative control: 10 μL non-relevant antibody 3. 2.5 μL antibody 4. 1 μL antibody
ELISA Monoclonal Anti-Bovine Serum Albumin, Clone: BSA-33 (Cat. No. B2901) was serially diluted for indirect ELISA using 5 μg/mL BSA for coating. The antibody was developed using Goat Anti-Mouse IgG (Fab specific)-Peroxidase (Cat. No. A9917) and a chromogenic substrate.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | BSA-33, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG2a |
| Quality Level | 200  |
| shipped in | dry ice |
| should not react with | human, hamster, rabbit, donkey, guinea pig, mouse, rat, chicken, feline, pigeon, pig |
| species reactivity | bovine (high), turkey (low), canine (low), sheep (high), horse (low), goat (high) |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: suitable |
| indirect ELISA: 1:1,000 |
| Application: | Monoclonal Anti-Bovine Serum Albumin has been used in: • enzyme linked immunosorbent assays (ELISA) • competitive ELISA • immunodot blot • western blotting • immunoaffinity purification or adsorption of BSA from biological fluids. |
| Biochem/physiol Actions: | Bovine serum albumin (BSA) is often used as a protein standard and can also be used in cell culture media, forensics, and foodstuffs preparation. It also acts as a transporter for drugs, hormones and fatty acids. |
| Biochem/physiol Actions: | Bovine serum albumin (BSA) is the main protein synthesized in the liver that constitutes over 50% of total serum proteins. Monoclonal Anti-Bovine Serum Albumin antibody is specific for SDS-denatured and reduced BSA. While the product is highly cross reactive with goat and sheep serum; it has a lower affinity for dog, turkey and horse serum albumins. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Bovine serum albumin (BSA) is a 66 kDa α-helical, globular and non-glycosylated protein. It is characterized with three domains with two subdomains under each. It belongs to the serum albumins family and has 17-disulfide bonds. |
| Immunogen: | Bovine serum albumin |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |