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BCIP®/NBT Liquid Substrate System

SIGMA/B1911 - ready to use solution

Synonym: 5-bromo-4-chloro-3-indolyl-phosphate / Nitro blue tetrazolium chloride solution; BCIP/NBT Alkaline Phosphatase Substrate solution

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-B1911-100ML 100 mL
 $111.00
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Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo-4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures.This substrate system produces an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually. Immunohistochemical staining with BCIP/NBT utilizes requires organic mounting media and can be counterstained with Nuclear Fast Red or Light Green.
A representative image of the product packaging

 

form liquid
Quality Level 300 
storage temp. 2-8°C
Application: A ready-to-use, precipitating substrate system for alkaline phosphatase. This substrate system produces an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually. Immunohistochemical staining with BCIP/NBT utilizes requires organic mounting media and can be counterstained with Nuclear Fast Red or Light Green. BCIP/NBT is generally a more sensitive method than Fast Red. Not recommended for ELISA (microwell) procedures.
Application: BCIP®/NBT Liquid Substrate System has been used:
• for staining alkaline phosphatase(ALP) in bone marrow stromal cells
• as a substrate in western blot detection of matrix metalloproteinase-2 specific antibody
• as a substrate for streptavidin-alkaline phosphatase in the enzyme-linked immunospot (ELISpot) assays
Disclaimer: It is recommended to store the product between 4 - 8 °C. Do not freeze. The product may be stored at room temperature for up to one year. If a precipitate forms, the solution can be gently swirled or filtered.
Legal Information: BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
Packaging: 100 mL in poly bottle
RIDADR NONH for all modes of transport
WGK Germany WGK 1
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. 2-8°C
UNSPSC 12352204

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