bisBenzimide H 33258
SIGMA/B1155 - powder, BioReagent, suitable for cell culture, ≥98% (HPLC and TLC)
Synonym: 2′-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi(1H-benzimidazole) trihydrochloride; 2-
CAS Number: 23491-45-4
Empirical Formula (Hill Notation): C25H24N6O · 3HCl
Molecular Weight: 533.88
EC Number: 245-690-6
MDL Number: MFCD00012679
Linear Formula: C25H24N6O · 3HCl
Product Type: Chemical
Immunofluorescence HeLa cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone. Fixed cells were stained with 1 μg/mL bisBenzimide H 33258 (Cat. No. B1155) to stain nuclei.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: B1155-100MG
| assay | ≥98% (HPLC and TLC) |
| form | powder |
| InChI | 1S/C27H28N6O.3ClH.H2O/c1- |
| InChI key | OWRSPPSBNWJJAR-UHFFFAOYSA |
| product line | BioReagent |
| Quality Level | 200  |
| SMILES string | Cl[H].Cl[H].Cl[H].[H]O[H] |
| solubility | H2O: 10 mg/mL |
| water and ethanol: 10 mg/mL | |
| storage temp. | −20°C |
| suitability | passes application test for fluorescence |
| technique(s) | cell culture | mammalian: suitable |
| Application: | bisBenzimide H 33258 is useful for staining DNA, chromosomes and nuclei. bisBenzimide H 33258 may be used for fluorescence microscopy or flow cytometry. Excitation max. = 346 nm Emission max. = 460 nm |
| Application: | bisBenzimide H 33258 is useful for staining DNA, chromosomes, and nuclei. May be used for fluorescence microscopy or flow cytometry. |
| Biochem/physiol Actions: | bisBenzimide H 33258 is a potent and selective Bcl-XL inhibitor. |
| Biochem/physiol Actions: | bisBenzimide H 33258 is a potent and selective Bcl-XL inhibitor. bisBenzimide H 33258 is a membrane-permeable, fluorescent DNA stain with low cytotoxicity that intercalate in A-T regions of DNA. |
| Packaging: | 100 mg in glass bottle |
| Packaging: | 25 mg in poly bottle |
| Hazard Codes | Xn |
| Risk Statements | 22-36/38 |
| Safety Statements | 26-36 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (HPLC and TLC) |
| Storage Temp. | −20°C |
| UNSPSC | 41106305 |