AccuTaq™ LA 10× Buffer
SIGMA/B0174 - 10X Buffer for long and accurate PCR
Product Type: Chemical
Longer products with improved fidelity with AccuTaq LA DNA polymerase Amplification of 2.5, 7, 9, 10, 12, and 15kb fragments of lambda DNA. 2.5 μg of DNA was amplified using 2.5 units of enzyme. The resulting products (6% of total reaction)were electrophoresed on a 0.8% agarose gel. Lanes 3-8 contained standard Taq DNA polymerase and Lanes 11-16 contained AccuTaq LA DNA polymerase. Lanes 1, 9. 1 kb DNA Marker 2, 10. Lambda Hind III DNA Marker 3, 11. 2.5 kb 4, 12. 7 kb
| concentration | 10 × |
| form | liquid |
| grade | Molecular Biology |
| packaging | vial of 0.5 mL |
| Quality Level | 200  |
| storage temp. | −20°C |
| technique(s) | PCR: suitable |
| Application: | AccuTaq™ LA 10× Buffer has been used: • in PCR reaction mixture for the amplification of human BRCA1 gene. • as a component of the PCR mix for the amplification of the genomic DNA from Triticum aestivum • as a component of the PCR mix for the reverse transcription of RNA from foot and mouth disease virus (FMDV) |
| General description: | AccuTaq™ LA 10X buffer is optimized for enhanced amplification of complex templates, when using AccuTaq long and accurate (LA) DNA polymerase. |
| Legal Information: | AccuTaq is a trademark of Sigma-Aldrich Co. LLC |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41106306 |