Anti-GATA2 antibody produced in rabbit
SIGMA/AV31855 - IgG fraction of antiserum
Synonym: Anti-GATA binding protein 2
Product Type: Chemical
Immunohistochemistry Anti-GATA2: Cat. No. AV31855: Immunohistochemistry of GATA2 in Human Liver tissue with GATA2 antibody at 4-8 μg/mL.
Immunoblotting Cell Type: k562 (Cat. No. AV31855). Lanes 1. Antibody dilution (concentration) 1.0-2.0 μg/mL in cell type k562
Immunoblotting GATA2: Cat. No. AV31855: Western blot analysis of GATA2 in Mouse liver, N2a cell lysates with GATA2 antibody at 2 μg/mL .
Immunoblotting GATA2 Antibody: Cat. No. AV31855: Western Blot analysis of GATA2 in Human Liver cell lysates with GATA2 Antibody at 1.0 μg/mL.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 50 kDa |
| NCBI accession no. | NP_116027   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rabbit, rat, guinea pig, dog, bovine, human, horse, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | P23769 |
| Application: | Rabbit Anti-GATA2 antibody can be used for western blot assays (1-2μg/ml) and immunohistochemical (4-8μg/ml, using paraffin embedded tissues) applications. |
| Application: | Rabbit polyclonal anti-GATA2 antibody is used to tag GATA binding protein 2 for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of GATA binding protein 2 in myeloid lineage determination, megakaryocyte development and hematopoietic stem/progenitor cell differentiation. |
| Biochem/physiol Actions: | The GATA family of transcription factors, which contain zinc fingers in their DNA binding domain, have emerged as candidate regulators of gene expression in hematopoietic cells is essential for normal primitive and definitive erythropoiesis and is expressed at high levels in erythroid cells, mast cells, and megakaryocytes. GATA2 is expressed in hematopoietic progenitors, including early erythroid cells, mast cells, and megakaryocytes, and also in nonhematopoietic embryonic stem cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | GATA binding protein 2 (GATA2) is a transcription factor involved in the regulation of ematopoiesis wherein it is essential for the regulation of myeloid lineage determination. GATA2 is required for normal megakaryocyte development and it is a negative regulator of hematopoietic stem/progenitor cell differentiation. GATA2 is a novel poor prognostic marker in pediatric AML. |
| General description: | Rabbit polyclonal anti-GATA2 antibody reacts with canine, chicken, bovine, pig, human, mouse, and rat GATA binding protein 2 transcription factors. |
| Immunogen: | Synthetic peptide directed towards the N terminal region of human GATA2 |
| Other Notes: | Synthetic peptide located within the following region: PYYANPAHARARVSYSPAHARLTGG |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |