Monoclonal Anti-VGLUT1 antibody produced in mouse
SIGMA/AMAB91041 - Prestige Antibodies® Powered by Atlas Antibodies, clone CL2754, purified immunoglobulin, buffered aqueous glycerol solution
Synonym: BNPI; SLC17A7
Product Type: Chemical
Enhanced Validation-Orthogonal RNAseq Confirmation Immunohistochemistry analysis in human cerebral cortex and tonsil tissues using AMAB91041 antibody. Corresponding VGLUT1 RNA-seq data are presented for the same tissues.
Immunohistochemistry Immunofluorescence staining of rat brain shows strong positivity in glutamatergic processes in the molecular and granular layers of cerebellum.
Immunohistochemistry Immunofluorescence staining of rat brain shows strong positivity in glutamatergic fibers in the polymorph layer of dentate gyrus.
Immunohistochemistry Immunofluorescence staining of mouse brain shows strong positivity in glutamatergic fibers in the molecular and granular layers of the cerebellum.
Immunohistochemistry Immunofluorescence staining of mouse hippocampus shows strong positivity in glutamatergic fibers in the CA2 layer.
Immunohistochemistry Immunohistochemical staining of human cerebral cortex shows strong positivity in neuropil.
Immunohistochemistry Immunohistochemical staining of human tonsil shows no positivity in lymphoid cells as expected.
Western Blotting Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Human Cerebral Cortex tissue
Prestige Antibodies® Highly characterized and Enhanced Validated antibodies
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | CL2754, monoclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| enhanced validation | orthogonal RNAseq Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | PSISEEERKYIEDAIGESAKLMNPL |
| isotype | IgG2b |
| pH range | 7.2-7.4 |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 1 μg/mL |
| immunohistochemistry: 1:5000- 1:10000 | |
| UniProt accession no. | Q9P2U7  |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Biochem/physiol Actions: | Vesicular glutamate transporter 1 (VGLUT1) plays a vital role as an vesicular glutamate transporter in glutamatergic neurons. VGLUT1 expression in the prefrontal cortex, functions as a potential neurochemical marker of dementia in Alzheimer disease (AD) and antidepressant activity. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | Vesicular glutamate transporter 1 (VGLUT1), also called as solute carrier family 17 member 7 (SLC17A7), is encoded by the gene mapped to human chromosome 19q13, a region associated with schizophrenia. VGLUT1 is specifically expressed in neuronal cells in the brain and at higher level in neuron-enriched regions such as the amygdala and hippocampus. Glia-enriched areas such as the corpus callosum also show moderate level of expression and substantia nigra, subthalamic nuclei and thalamus show low level expression. VGLUT1 is characterized with a conserved C-terminal dileucine-like motif and two polyproline domains distal to C- terminal end, including one that binds to endocytic BAR (Bin/Amphiphysin/Rvs) domain protein, endophilin. |
| Immunogen: | solute carrier family 17 member 7 |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST70070 |
| Physical form: | 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. |
| Preparation Note: | Upon arrival, mix gently and aliquot to avoid repeated freeze-thaw cycles |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |