Aminopeptidase I from Streptomyces griseus
SIGMA/A9934 - lyophilized powder, ≥200 units/mg protein
Synonym: Leucine Aminopeptidase IV
CAS Number: 9031-94-1
EC Number: 232-874-6
MDL Number: MFCD00166325
Product Type: Chemical
Unit Definition One unit will hydrolyze 1.0 μmole of L-leucine p-nitroanilide to L-leucine and p-nitroaniline per min at pH 8.0 at 25 °C.
| composition | Protein, 40-60% Lowry |
| form | lyophilized powder |
| mol wt | 21 kDa by gel filtration |
| 33 kDa by SDS-PAGE | |
| Quality Level | 200  |
| specific activity | ≥200 units/mg protein |
| storage temp. | −20°C |
| Application: | Aminopeptidase I from Streptomyces griseus has been used: • to test the biochar exposure effect on the enzyme activity • in circular dichroism (CD) spectroscopy studies • as a positive control in p-nitroanilide degradation assay |
| Biochem/physiol Actions: | Aminopeptidase I from S. griseus has a fairly broad specificity, being able to remove the N-terminal residue of most proteins, except where the penultimate residue is an imino acid. It contains two Zn2+ binding sites. Aminopeptidase I from S. griseus is inhibited by 1,10-phenanthroline and is activated six-fold by Ca2+, which also stabilizes it against heat inactivation. This monomeric zinc metalloprotein has an isoelectric point (pI) of 5.4. |
| Biochem/physiol Actions: | Aminopeptidase I may also be used as a reagent in the assay of endoprotease activities with a synthetic substrate in a two-stage assay. In the first stage, the endoprotease cleaves a peptide, such as Z-Y-X-Leu-p-nitroanilide, with the X, Y, and Z residues being chosen according to the specificity of the endoprotease. |
| General description: | Aminopeptidase I from Streptomyces griseus is a thermostable enzyme with Glu131 and Tyr246 as key active site residues. |
| Other Notes: | Endopeptidase contaminant: Not more than: 0.01 U/mg protein (as μmole tyrosine equivalent per min released from casein.) |
| Other Notes: | One unit will hydrolyze 1.0 μmole of |
| Packaging: | 0.1 mg in serum bottle |
| Packaging: | Package size based on protein content. |
| Physical form: | Contains calcium acetate |
| Preparation Note: | Reconstitute in 20 mM tricine, pH 8.0, with 0.05% bovine serum albumin. Dilute the enzyme with the reconstitution buffer to 0.15-0.3 U/mL for a working concentration. Solutions should be prepared fresh prior to use. |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H334 - H335 |
| Precautionary statements | P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥200 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.11.22 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |