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Auramine O

SIGMA/A9655 - Dye content ≥80 %, certified by the BSC

Synonym: 4,4′-(Imidocarbonyl)bis(N,N-dimethylaniline) monohydrochloride; Basic Yellow 2; Pyoctaninum aureum

CAS Number: 2465-27-2
Empirical Formula (Hill Notation): C17H21N3 · HCl
Molecular Weight: 303.83
EC Number: 219-567-2
MDL Number: MFCD00012484
Linear Formula: C17H21N3 · HCl
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-A9655-25G 25 g
 $87.30
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This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A9655-25G

 

λmax 370 nm in H2O
  432 nm in H2O
agency certified by the BSC
application(s) diagnostic assay manufacturing
hematology
histology
color yellow to green
composition Dye content, ≥80%
form powder
InChI 1S/C17H21N3.ClH/c1-19(2)15-9-5-13(6-10-15)17(18)14-7-11-16(12-8-14)20(3)4;/h5-12,18H,1-4H3;1H
InChI key KSCQDDRPFHTIRL-UHFFFAOYSA-N
mp >250 °C (dec.) (lit.)
Quality Level 200 
SMILES string Cl[H].CN(C)c1ccc(cc1)C(=N)c2ccc(cc2)N(C)C
solubility ethanol: 1 mg/mL, yellow to orange
storage temp. room temp
technique(s) microbe id | staining: suitable
Application: Auramine O has been used to study its absorption by a natural biopolymer poly (γ-glutamic acid).
General description: Certified for use by fluorescence microscopy in Churukian′s modification of Truant′s fluorescent method for acid fast bacilli on paraffin sections.

Auramine O is used for the staining of acid-fast organisms like coccidia. Along with carbol, it generates brilliant yellow fluorochrome of tubercle bacilli. It is effective in detecting positive cases of tuberculosis. Auramine O binds to the mycolic acid in the bacterial cell wall.
Packaging: 25 g in glass bottle
mp >250 °C (dec.) (lit.)
Storage Temp. room temp
Colour Index Number 41000
UNSPSC 12171500

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