Amphotericin B solubilized

SIGMA/A9528 - powder, γ-irradiated, BioXtra, suitable for cell culture

Synonym: Amphotericin B from Streptomyces sp.; Fungizone

CAS Number: 1397-89-3
Empirical Formula (Hill Notation): C₄₇H₇₃NO₁₇
Molecular Weight: 924.08
MDL Number: MFCD00877763
Linear Formula: C₄₇H₇₃NO₁₇
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-A9528-50MG	50 mg	$55.20 1/EA	Add To Favorites
45-A9528-100MG	100 mg	$100.00 1/EA	Add To Favorites
45-A9528-500MG	500 mg	$618.00 1/EA	Add To Favorites
45-A9528-1G	1 g	$1110.00 1/EA	Add To Favorites
45-A9528-5G	5 g	$4030.00 1/EA	Add To Favorites

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A9528-100MG

Properties
Descriptions
Safety Info.
Basic Data

antibiotic activity spectrum	fungi
	yeast
form	powder
InChI	1S/C47H73NO17/c1-27-17-15-13-11-9-7-5-6-8-10-12-14-16-18-34(64-46-44(58)41(48)43(57)30(4)63-46)24-38-40(45(59)60)37(54)26-47(61,65-38)25-33(51)22-36(53)35(52)20-19-31(49)21-32(50)23-39(55)62-29(3)28(2)42(27)56/h5-18,27-38,40-44,46,49-54,56-58,61H,19-26,48H2,1-4H3,(H,59,60)/b6-5+,9-7+,10-8+,13-11+,14-12+,17-15+,18-16+/t27-,28-,29-,30+,31+,32+,33-,34-,35+,36+,37-,38-,40+,41-,42+,43+,44-,46-,47+/m0/s1
InChI key	APKFDSVGJQXUKY-INPOYWNPSA-N
mode of action	cell membrane \| interferes
product line	BioXtra
Quality Level	300
SMILES string	C[C@H]1O[C@@H](O[C@@H]2C[C@@H]3O[C@](O)(C[C@@H](O)C[C@@H](O)[C@H](O)CC[C@@H](O)C[C@@H](O)CC(=O)O[C@@H](C)[C@H](C)[C@H](O)[C@@H](C)C=CC=CC=CC=CC=CC=CC=C2)C[C@H](O)[C@H]3C(O)=O)[C@@H](O)[C@@H](N)[C@@H]1O
sterility	γ-irradiated
storage temp.	2-8°C
technique(s)	cell culture \| mammalian: suitable

Application:	Amphotericin B is a polyene antifungal antibiotic from Streptomyce sp. It has a high affinity for sterols, especially ergosterols, of both fungal and bacterial cell membranes. Normal usage in maintenance of antifungal cell cultures is 2.5 mg/L with penicillin and streptomycin used in the medium.
Biochem/physiol Actions:	Amphotericin B binds to sterols, forming pores in the membrane, and causing small molecules to leak out. The product is most effective against fungi and yeast.
Disclaimer:	Amphotericin B remains active for 3 days in culture at 37°C. At -20°C, and with protection from air and light, the product remains active for 5 years.
General description:	Chemical structure: polyene
Other Notes:	After reconstitution with water, the product will be a suspension of Amphotericin B, containing deoxycholate as the solubilizing agent. The lyophilized formulation is ~45% Amphotericin B and 35% sodium deoxycholate in sodium phosphate and sodium chloride.
Other Notes:	Keep container tightly closed in a dry and well-ventilated place. Product is sensitive to light and moisture. Keep in a dry place.
Other Notes:	The actual solid weight of the packaged product is calculated according to the UV purity.
Packaging:	1, 5 g in glass bottle
Packaging:	50, 100, 500 mg in glass bottle
Packaging:	Solubilized Amphotericin B is packaged in amber glass bottle.
Preparation Note:	Amphotericin B is soluble at 19.6-20.4 mg/ml in water, at natural pH without heating, giving a yellowish clear to slightly hazy solution. Aqueous solutions cannot be sterile filtered due to poor solubility. If reconstituted in sterile water, there is no need to filter sterilize. It is also soluble in DMSO (30-40 mg/mL) and in dimethylformamide (2-4 mg/mL).

Symbol	GHS07
Signal word	Warning
Hazard statements	H335
Hazard Codes	Xn
Risk Statements	22-36/37/38
Safety Statements	26
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	2-8°C
UNSPSC	12352207
Components	Amphotericin B ~ 45 %; Sodium deoxycholate ~ 35 %; Sodium phosphate balance

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