Acetylcholine chloride
SIGMA/A9101 - pkg of 150 mg (per vial)
Synonym: ACh
CAS Number: 60-31-1
Empirical Formula (Hill Notation): C7H16ClNO2
Molecular Weight: 181.66
EC Number: 200-468-8
MDL Number: MFCD00011698
Linear Formula: (CH3)3N+CH2CH2OCOCH3Cl-
Product Type: Chemical
| assay | ≥99% (TLC) |
| form | powder or crystals |
| InChI | 1S/C7H16NO2.ClH/c1-7(9)10 |
| InChI key | JUGOREOARAHOCO-UHFFFAOYSA |
| mp | 146-150 °C (lit.) |
| packaging | pkg of 150 mg (per vial) |
| Quality Level | 100  |
| SMILES string | [Cl-].CC(=O)OCC[N+](C)(C) |
| solubility | H2O: soluble |
| storage temp. | room temp |
| Application: | For use as a substrate to determine acetylcholinesterase activity. Acetylcholine chloride has been used: • for standard curve preparation in hestrin assay • in Protein expression, radioactive ligand binding assay and electrophysiological studies of Lymnaea stagnalis acetylcholine binding protein (Ls-AChBPs) • as a supplement in extracellular solutions for treating the voltage clamped cells in electrophysiology studies |
| Biochem/physiol Actions: | Acetyl choline (ACh) is an essential neurotransmitter in both central and peripheral nervous system. ACh binds to nicotinic acetylcholine receptors (nAChRs) and causes fast excitatory neurotransmission. |
| Biochem/physiol Actions: | Acetylcholine chloride, injected at 20 mg/kg body weight, reduces mortality and plasma proinflammatory cytokines in mice with experimentally-induced sepsis . The cholinergic anti-inflammatory mechanism is probably mediated by interaction of acetylcholine with α7n cholinoreceptor on monocytes, macrophages, and neutrophils, which decreases the levels of proinflammatory cytokines such as TNF-α, IL-1β, and IL-6. |
| Biochem/physiol Actions: | Endogenous neurotransmitter at cholinergic synapses; amplifies action potential of the sarcolemma thereby inducing muscle contractions. |
| General description: | Acetylcholine is an important neurotransmitter that plays a critical role in multiple neurological systems. Crucial for neurobiology studies, acetylcholine aids researchers in understanding synaptic transmission. In other forms of research, acetylcholine has an effect and influence on tumor neurobiology for cancer R&D, as well as a variety of other cognitive processes. |
| Preparation Note: | Reconstituted solution is stable for 2 weeks when stored at 2-8 °C |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99% (TLC) |
| mp | 146-150 °C (lit.) |
| Storage Temp. | room temp |
| UNSPSC | 12352204 |