Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in rabbit
SIGMA/A9044 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti Mouse Antibody; Anti Mouse Antibody - Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in rabbit; Anti Mouse Hrp; Anti Mouse Hrp Sigma; Anti Mouse Igg; Sigma Anti Mouse Hrp
MDL Number: MFCD00162644
Product Type: Chemical
Immunohistochemistry Formalin-fixed, paraffin-embedded human appendix sections stained with Monoclonal Anti-Actin, α-Smooth Muscle (Cat. No. A2547) followed by Anti-Mouse IgG (whole molecule)-Peroxidase antibody produced in rabbit (Cat. No. A9044).
Western Blotting Whole extract of HS-68 human foreskin fibroblast cells were separated on SDS-PAGE and probed with Monoclonal Anti-α-Tubulin antibody produced in Mouse, Clone: B-5-1-2 (Cat. No. T6074). The antibody was developed using Anti-Mouse IgG (whole molecule)-Peroxidase antibody produced in Rabbit (Cat. No. A9044). Lanes 1. 1:80,000 antibody 2. 1:40,000 antibody 3. Negative Control
| antibody form | IgG fraction of antiserum |
| antibody product type | secondary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | peroxidase conjugate |
| form | buffered aqueous solution |
| Quality Level | 300  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:40,000 using using 5 μg/ml of mouse IgG for coating and OPD substrate |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 | |
| western blot (chemiluminescent): 1:80,000-160,000 |
| Application: | Anti-Mouse IgG (whole molecule)–Peroxidase antibody has been used in western blotting , immunohistochemistry and protein pin array assay. |
| Application: | Anti-Mouse IgG (whole molecule)–Peroxidase antibody may be used in direct ELISA at a working antibody dilution of 1:40,000. For immunoblotting, a dilution of 1:80,000 to 1:160,000 may be used. A working antibody dilution of 1:200 is recommended for immunohistochemistry of formalin-fixed, paraffin-embedded human tonsil or human appendix sections. Mouse IgG-peroxidase antibody was used at a dilution of 1:20 for immunohistochemistry of mice muscle tissue sections; a dilution of 1:500 was used for South-western blotting in the same study. For immunoblotting of human skeletal muscle tissue extracts, a dilution of 1:5000 was used. |
| Application: | Epidermis protein extracts were generated from human mammary surgery specimens and subjected to western blot using HRP-conjugated rabbit anti-mouse IgG as the secondary at a 1:80000 dilution in TBSt/5% milk. |
| Biochem/physiol Actions: | Anti-Mouse IgG (whole molecule)-Peroxidase antibody is specific for all mouse IgG subclasses. |
| Biochem/physiol Actions: | IgG antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. IgG antibody has its function similar to IgM antibody in complement system activation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids. IgG antibody is a glycoprotein, composed of two polypeptide chains (light and heavy), each possessing variable and constant domains. The variable region of IgG antibody is specific to antigens and is highly conserved. Anti-Mouse IgG (whole molecule)-Peroxidase antibody is specific for all mouse IgG subclasses. IgG is purified from mouse serum by fractionation and ion exchange chromatography. The purified IgG is then conjugated to peroxidase by protein cross-linking with 0.2% glutaraldehyde. |
| General description: | Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by disulfide bond. It mainly helps in immune defense. It is a glycoprotein and a major class of immunoglobulin. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity. |
| Immunogen: | Purified mouse IgG |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT |
| Preparation Note: | Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978). |
| Preparation Note: | Store at -20 °C for long term. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots -20 °C. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |