Anti-Human Kappa Light Chains (Bound and Free)−Peroxidase antibody produced in goat
SIGMA/A7164 - affinity isolated antibody, buffered aqueous solution
Synonym: Goat Anti-Human Kappa Light Chains (Bound and Free)−HRP
MDL Number: MFCD00162521
Product Type: Chemical
Western Blotting JOURNAL CITATION: Antibody degradation in tobacco plants: a predominantly apoplastic process. By: Hehle, V. K., Paul, M. J., et al. in BMC Biotechnol, 2011. PubMed ID: 22208820 Image collected and cropped by CiteAb from the following publication, (http://bmcbiotechnol.biomedcentral.com/articles/10.1186/1472-6750-11-128), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Antibody degradation in tobacco plants: a predominantly apoplastic process. By: Hehle, V. K., Paul, M. J., et al. in BMC Biotechnol, 2011. PubMed ID: 22208820 Image collected and cropped by CiteAb from the following publication, (http://bmcbiotechnol.biomedcentral.com/articles/10.1186/1472-6750-11-128), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Antibody degradation in tobacco plants: a predominantly apoplastic process. By: Hehle, V. K., Paul, M. J., et al. in BMC Biotechnol, 2011. PubMed ID: 22208820 Image collected and cropped by CiteAb from the following publication, (http://bmcbiotechnol.biomedcentral.com/articles/10.1186/1472-6750-11-128), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Antibody degradation in tobacco plants: a predominantly apoplastic process. By: Hehle, V. K., Paul, M. J., et al. in BMC Biotechnol, 2011. PubMed ID: 22208820 Image collected and cropped by CiteAb from the following publication, (http://bmcbiotechnol.biomedcentral.com/articles/10.1186/1472-6750-11-128), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | peroxidase conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:1,000 |
| Application: | Anti-Human κ Light Chains (Bound and Free)-Peroxidase antibody is suitable for use in western blot (1:1000). |
| Application: | ELISA Assays were performed using HRP-conjugated goat anti-human kappa light chains IgG to detect IgG1 and free K chains in cultured media of human LCL cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Human κ light chains are antibody fragments that regulate immunological responses in cells. Increased expression of κ light chains has been linked to chronic lymphocytic leukemia . The variable fragment of the κ light chain gene has also been associated with vaccine-induced antibody response to the polysaccharide coat in Haemophilus influenzae type b . Anti-Human κ Light Chains (Bound and Free)-Peroxidase antibody detects human κ (bound and free) light chains when tested against human IgA, IgG, IgM, and Bence Jones κ and λ myeloma proteins. |
| Immunogen: | Purified human κ light chains |
| Packaging: | 1 mL in glass bottle |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin with preservative. |
| Preparation Note: | Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, p215, (Elsevier/North Holland Biomedical Press, Amsterdam, 1978). |
| Symbol |   GHS07,GHS09 |
| Signal word | Warning |
| Hazard statements | H315 - H317 - H319 - H411 |
| Precautionary statements | P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |